Bax抑制肽对新生大鼠肠组织的保护作用  

Protection of Bax inhibitory peptides to newborn rat intestinal tissue

作　　者：崔伟伦 冯帼 刘素丽 谭洪辉 叶炳飞[2] 张炼 

机构地区：[1]广州市番禺区中心医院,广州511486 [2]广州医科大学实验动物中心,广州510182 [3]广州市妇女儿童医疗中心,广州510623

出　　处：《广州医药》2015年第6期12-17,共6页Guangzhou Medical Journal

基　　金：广东省科技计划项目[粤科规划字(2013)137号]

摘　　要：目的观察Bax抑制因子(Bax-inhibiting peptides,BIP)对肠上皮细胞的保护作用,并探讨其作用机制。方法建立1日龄新生大鼠的坏死性小肠结肠炎模型。BIP于建模前腹腔内注射,建模后分别检测各组肠组织病理,并分别用流式细胞仪检测各组肠细胞凋亡率,western blot法检测bax下游凋亡蛋白细胞色素C、caspase 9和caspase 3含量。结果与NEC组及Bax10μg组比较,bax50μg和100μg可减轻肠上皮损伤,减少肠细胞凋亡率,降低bax下游凋亡蛋白细胞色素C、caspase9和caspase3含量。结论一定剂量的Bax抑制肽可通过降低活性Caspase-3及Cyt C蛋白释放,保护线粒体膜,抑制肠上皮细胞调亡,发挥对肠上皮细胞的保护作用。Objective To investigate the protective effects of Bax-inhibiting peptides（ BIP） on intestinal epithelial cells and to explore its mechanism. Methods The model of neonatal necrotizing enterocolitis in 1 day neonatal rats were established. Before establishing the model,BIP was intraperitoneal injected to the rats. The pathological of intestinal tissue were detected respectively,the intestinal cell apoptosis rate were detected by flow cytometry,the levels of downstream apoptosis proteins of Bax were detected by Western blotting respectively. Results Compared to NEC and Bax10μg group,bax50μg and 100 μg can significantly reduce the intestinal epithelial damage and intestinal cell apoptosis rate and can decrease the levels of cytochrome C,caspase-9 and caspase-3,downstream apoptosis proteins of Bax,significantly. Conclusion A certain dose of Baxinhibiting peptides can protect the mitochondrial membrane,inhibit the apoptosis of intestinal epithelial cells by reducing the level of Caspase-3 and Cyt C and play a protective effect on intestinal epithelial cells.

关 键 词：Bax抑制肽 肠上皮细胞凋亡 坏死性小肠结肠炎 保护作用 

分 类 号：R722.1[医药卫生—儿科]