甜瓜蔓枯病病原菌分离与分生孢子的产孢诱导  

Isolation and Identification of Pathogen of Didymella Bryoniae and Spore Production Induction of Condium

作　　者：张立杰[1] 张慧玲[1,2] 哈矿武[1,2] 王建设[2] 

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]国家蔬菜工程技术研究中心,北京100097

出　　处：《宁夏大学学报（自然科学版）》2015年第4期382-385,共4页Journal of Ningxia University(Natural Science Edition)

基　　金：北京市自然科学基金资助项目(5052012;6092008)

摘　　要：实验成功分离甜瓜蔓枯病病原菌.菌丝体在马铃薯蔗糖培养基(PSA)上不易产孢,在易产孢的燕麦培养基(OA)、玉米培养基(MA)和马铃薯燕麦葡萄糖培养基(POGA)上,仅能产生极少量分生孢子器.而采用热激和紫外照射却能高效诱导出大量的分生孢子器.在以分生孢子器扩繁菌种时,发现易产孢的3种培养基上产生大量的分生孢子器,而在PSA上仍然不能.The experiment successfully extracted the pathogenic bacteria from didymella bryoniae.The mycelium is hard to produce spore on potato sucrose Medium,and can produce very few of pycnidium on oatmeal agar,corn meal medium,and potato oat dextrose agar which are easily produce spore.Methods of heat shock and ultraviolet radiation,however,can efficiently induce a large quantity of pycnidium.Three above mediums which can easily produce spore can produce a lot of pycnidium,but the potato sucrose medium,can’t when expanding propagation bacteria with pycnidium.

关 键 词：甜瓜 蔓枯病 病原分离 分生孢子诱导 

分 类 号：S652[农业科学—果树学]