云南松松塔多糖含量测定两种方法的比较  

Comparison of Two Methods of Determination in the Pinecone of Pinus yunnanensis

作　　者：王莹[1,2] 张海珠[1,2] 刘熙[1,2] 李杨[1] 

机构地区：[1]大理学院药学与化学学院,云南大理671000 [2]云南省昆虫生物医药研发重点实验室,云南大理671000

出　　处：《食品研究与开发》2015年第19期145-147,共3页Food Research and Development

基　　金：国家自然科学基金(81260632);云南省教育厅重点项目(云南松松塔多糖的分离纯化及免疫活性研究);大理学院应用开发科研基金(KYYY201304)

摘　　要：通过对云南松松塔中多糖的含量的测定,比较硫酸-蒽酮和硫酸-苯酚两种常用多糖测量方法。通过精密度试验、稳定性试验、回收率试验等方面,对硫酸-蒽酮和硫酸-苯酚两种常用多糖测量方法进行比对。结果表明:硫酸-苯酚法在云南松松塔多糖含量测定中较硫酸-蒽酮法优越,硫酸-苯酚法测定云南松松塔多糖含量时,操作简便,精密度、稳定性高,为云南松松塔等植物的多糖含量测定提供了参考。Determination of the content in the cloud Nansongsongta polysaccharides, more sulfuric acid-anthrone and sulfuric acid- phenol two common polysaccharide measurement method. By aspects of precision test, stability test, recovery test of sulfuric acid, sulfuric acid-anthrone and sulfuric acid- phenol two common polysaccharide measurement methods were compared. Sulfuric acid-phenol method in the determination of the polysaccharide content cloud Nansongsongta than sulfuric acid-excellent anthrone method. Sulfuric acid-the determination of the polysaccharide content cloud Nansongsongta phenol method, simple operation, high precision, stability, and other plants for the determination of the cloud Nansongsongta polysaccharide content to provide a reference.

关 键 词：云南松 松塔 硫酸-苯酚法 硫酸-蒽酮法 多糖含量 

分 类 号：R284.1[医药卫生—中药学]