毛果杨PGR5基因转南林895杨的分子鉴定及表达量  

Molecular Detection and the Expression of PGR5 Gene in the Transgenic Poplars

作　　者：王丽娜[1] 李开隆[1] 

机构地区：[1]林木遗传育种国家级重点实验室(东北林业大学),哈尔滨150040

出　　处：《东北林业大学学报》2015年第11期6-8,共3页Journal of Northeast Forestry University

基　　金：国家科技支撑项目(2012BAD21B02)

摘　　要：采用农杆菌介导法将毛果杨（Populus trichocarpa）PGR5基因转化南林895杨,经潮霉素抗性基因筛选并对转基因植株进行了分子检测和表达量分析。普通PCR检测结果显示,有12株检测到阳性信号,表明该基因已整合到南林895杨基因组中;经RT-PCR检测,该12株转化子在目的基因处有阳性条带,表明该基因在转录水平表达;实时荧光定量PCR检测结果显示,12株阳性苗的表达量为野生型的2-20倍。We introduced PGR5 gene from Populus trichocarpa to Populus×euramericana cv. ‘Nanlin895 by agrobacterium mediated method,screened these transgenic plants by hygromycin resistance gene,and took the molecular detection and expression analysis of these transgenic plants. With the data of PCR detection,12 of 23 clones were positives,and the gene was integrated into the genome of poplars. By RT-PCR detection,12 clones had positive bands which were consistent with the anticipated objective strap size,and the gene was expressed at the transcripional level. By real time-PCR detection,the expression of the 12 positive clones were 220 times as much as wild types.

关 键 词：PGR5 分子检测 实时荧光定量PCR 转基因杨树 

分 类 号：S792.11[农业科学—林木遗传育种] Q78[农业科学—林学]