4种矮牵牛叶片直接诱导不定芽再生体系建立  

Establishing Adventitious Bud Induction and Regeneration System Directly from Leaves of Four Cultivars of Petunia hybrids

作　　者：平璐[1] 顾德峰[1] 韦正乙[2] 仲晓芳[2] 王云鹏[2] 林春晶[2] 邢少辰[2] 

机构地区：[1]吉林农业大学,长春130118 [2]吉林省农业科学院农业生物技术研究所

出　　处：《东北林业大学学报》2015年第11期14-21,共8页Journal of Northeast Forestry University

基　　金：吉林省科技发展计划项目(20076021;C42070501)

摘　　要：选择4种不同花色的矮牵牛品种‘Pink’、‘Red’、‘Pink Vein’和‘Peppermint’,以MS培养基为基础培养基,分别添加不同质量浓度的6-BA、NAA、IBA激素,除草剂双丙氨膦和抗生素壮观霉素,研究其不定芽的诱导频率,为后续的叶绿体转化提供受体。结果表明:影响矮牵牛叶片诱导不定芽因素中,品种间的影响比激素更重要。当诱导培养基中的6-BA质量浓度为1.0 mg·L-1、NAA质量浓度为0.5 mg·L-1时,诱导效果最佳,是品种‘Pink’、‘Pink Vein’的最适培养基;同样地,品种‘Peppermint’最适培养基中,6-BA质量浓度不变、NAA的质量浓度降低至0.3 mg·L-1;品种‘Red’最适培养基中,6-BA质量浓度仍不变、NAA质量浓度降低至0.1 mg·L-1。4个矮牵牛品种的不定芽诱导率,由高到低的顺序为‘Pink’、‘Pink Vein’、‘Peppermint’、‘Red’,因此,优先选择品种‘Pink’作为转基因受体材料。分别利用不同质量浓度双丙氨膦和壮观霉素筛选,通过二者对品种‘Pink’叶片分化的影响,发现双丙氨膦和壮观霉素的最低抑制质量浓度分别为3、100 mg·L-1。该研究结果对今后利用叶绿体遗传转化技术改良矮牵牛品种提供了技术支持。With four Petunia hybrids cultivars with different color involving‘Pink＇,‘Red＇,‘Pink Vein＇and‘Peppermint＇,we studied the frequency of adventitious bud induction and then subsequently provided explants for chloroplast transformation. The MS medium was served as basic medium supplemented with different concentrations of 6-BA,NAA and IBA,respectively,with the addition of bialaphos sodium and spectinomycin. The impact of cultivar on induction of adventitious buds from leaves of Petunia hybrid is more visible than that of hormone. The induction frequency of adventitious buds will be highest when the media were added with concentration of 1.0 mg·L^-16-BA and 0.5 mg·L^-1NAA for ‘Pink＇and‘Pink Vein＇. Similarly,the concentration of NAA will be lower to 0.3 mg·L^-1for ‘Peppermint＇and 0. 1 mg·L^-1for‘Red＇,while the concentration of 6-BA keep the same as above in the optimal medium. According to the order from high to low,the ranking of frequencies of adventitious bud induction for four cultivars is‘Pink＇,‘Pink Vein＇,‘Peppermint＇and‘Red＇.‘Pink＇,therefore,is considered to be the suitable explant for the genetic transformation. The minimum inhibitory concentration of bialaphos-sodium and spectinomycin in induction medium for ‘Pink＇is 3 mg · L^-1and 100 mg ·L^-1,respectively. Our results will provide technical support for chloroplast transformation of Petunia hybrid to implement genetic improvement.

关 键 词：矮牵牛 不定芽诱导 叶绿体转化 高频再生 

分 类 号：S681.6[农业科学—观赏园艺] Q943.1[农业科学—园艺学]