桦褐孔菌菌丝体液体培养条件的优化  

Optimization of Liquid Culture Conditions of Inonotus obliquus

作　　者：毛庆莲 刘雪峰[1] 董爱荣[1] 宋庆超[2] 

机构地区：[1]东北林业大学,哈尔滨150040 [2]大兴安岭地区塔河林业局绣峰林场

出　　处：《东北林业大学学报》2015年第11期119-121,共3页Journal of Northeast Forestry University

基　　金：国家林业局公益性项目(201004079)

摘　　要：应用单因素梯度法对采自黑龙江省大兴安岭呼中国家级自然保护区的桦褐孔菌(Inonotus obliquus)菌丝体的液体培养条件进行了优化研究,以真菌多糖产量和菌丝生物量为评价指标。结果表明:桦褐孔菌液体培养的最佳碳源为葡萄糖、最佳氮源为蛋白胨、最适培养温度27℃、最佳初始p H=7.5,最适摇培转速130 r·min-1。With the fruiting body of Inonotus obliquus collected from Huzhong National Nature Reserve of the Daxing ＇an Mountains of Heilongjiang Province,we studied the optimization of liquid culture conditions. Taking polysaccharide yield and biomass as two indexes,the liquid culture conditions were optimized using single factor gradient method,and the glucose and peptone were assigned to be the optimum carbon and nitrogen sources,respectively,with optimum temperature of27 ℃,optimum initial pH of 7.5,and 130 rpm of optimum speed for shaking culture.

关 键 词：桦褐孔菌 液体培养 多糖产量 菌丝生物量 

分 类 号：S718.8[农业科学—林学]