脑络欣通方对脑缺血大鼠血管再生及局部脑血流量的影响  

Influence of Naoluo Xintong Prescription on Angiogenesis and r CBF in MCAO/R Rats

作　　者：谭辉[1] 王键[1] 尹婷婷[1] 胡建鹏[1] 何玲[1] 徐伟[1] 

机构地区：[1]安徽中医药大学新安医学教育部重点实验室,合肥230038

出　　处：《中国实验方剂学杂志》2015年第22期21-25,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2012BAI26B00);国家中医药管理局安徽新安王氏内科流派传承工作室建设项目;安徽中医药大学自然科学研究基金项目(2014zr001)

摘　　要：目的:探讨脑络欣通方对大脑中动脉闭塞模型大鼠缺血再灌注(MCAO/R)损伤后大鼠血管新生及局部脑血流量(r CBF)的影响。方法:Wistar大鼠90只随机分为假手术组、模型组和脑络欣通组,采用线栓法制作MCAO/R模型,每组分为3,7,14 d共3个时间点的亚组,酶联免疫吸附试验(ELISA)检测脑组织匀浆中内皮素(ET)和血小板生成素(TPO)水平,免疫组化法检测脑组织中血管内皮生长因子(VEGF)的表达,运用激光多普勒仪动态观察r CBF。结果:与假手术组比较,模型组TPO和ET水平明显升高,VEGF蛋白表达增加,r CBF降低;与模型组比较,脑络欣通组TPO和ET水平显著降低,VEGF蛋白表达增加,r CBF明显恢复;同组内比较,脑络欣通组第7天和第14天较第3天TPO水平显著下降,第7天和第14天ET水平较第3天明显下降,第14天较第3天VEGF蛋白表达增加,第14天r CBF较第3天显著增加。结论:脑络欣通冻干粉通过调节模型大鼠凝血纤溶调节因子和VEGF,促进血管新生而改善血流状态,提高局部脑血流量来发挥对缺血性脑血管病的治疗作用。Objective： To discuss influence of Naoluo Xintong prescription on angiogenesis and r CBF in MCAO / R rats. Method： Wistar rats were randomly divided into sham group,model group and Naoluo Xintong group,model of MCAO / R rats was reproduced in accordance to suture method,and further were subdivided into three time points,such as 3,7,14 d. The content of thrombopoietin（ TPO） and endothelin（ ET） in brain tissue homogenate were determined by ELISA. Expressing of vascular endothelial growth factor（ VEGF） protein in cerebrum was assayed by immunohistochemistry. The r CBF was dynamically measured by laser Doppler flowmeter.Result： Compared with sham group,the content of TPO and ET,VEGF protein significantly increased,r CBF reduced in Naoluo Xintong group. Compared with model group,the content of TPO and ET markedly reduced,the amount of VEGF protein and r CBF increased in Naoluo Xintong group. Compared within the same group,the content of TPO in Naoluo Xintong group in 7 d and Naoluo Xintong group in 14 d markedly reduced by comparing with Naoluo Xintong group in 3 d,the content of ET in Naoluo Xintong group in 7 d and Naoluo Xintong group in 14 d markedly reduced by comparing with Naoluo Xintong group in 3 d. The amount of VEGF protein in Naoluo Xintong group in 14 d increased by comparing with Naoluo Xintong group in 3 d. The r CBF in Naoluo Xintong group in 14 d markedly increased by comparing with Naoluo Xintong group in 3 d. Conclusion： Naoluo Xintong lyophilized powder can improve blood flow status,increase r CBF by regulating regulatory factor related to cruor,fibrinolysis,VEGF and promoting angiogenesis,which play an treatment role on ischemic cerebral vascular disease（ ICVD）.

关 键 词：脑络欣通方 血小板生成素 内皮素 血管内皮生长因子 局部脑血流量 

分 类 号：R285.5[医药卫生—中药学]