拜颤停复方对帕金森病小鼠模型氧化应激反应的影响  

Effect of Baichanting Compound on Oxidative Stress Responses in Mice Model of Parkinson's Disease

作　　者：任燕冬[1,2] 井月娥[1] 张淑香[1] 王洪玉[1] 卢芳[1] 刘树民[1] 

机构地区：[1]黑龙江中医药大学,哈尔滨150040 [2]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007

出　　处：《中国实验方剂学杂志》2015年第22期154-157,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金青年基金项目(81303249);黑龙江省博士后基金项目(LBH-Z11017)

摘　　要：目的:通过研究拜颤停复方(BCT)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)致小鼠帕金森病(PD)模型神经行为学、纹状体多巴胺(DA)含量、氧化应激反应的影响,探讨BCT对PD模型神经保护作用的可能作用机制。方法:雄性C57BL/6小鼠,MPTP-HC1(30 mg·kg-1·d-1,ip,0.9%生理盐水溶解)5 d,造成PD小鼠模型;设立正常组、模型组、多巴丝肼组(62.50 mg·kg-1·d-1)及BCT高、中、低剂量组(363.00,181.50,95.75 mg·kg-1·d-1),各给药组均用0.5%羧甲基纤维素钠(CMC-Na)溶解;BCT高、中、低剂量组ig给药治疗20 d后,进行爬杆实验和自主活动实验以观察小鼠行为学的变化;采用UPLC-MS-MS三重四级杆串联质谱技术检测小鼠纹状体多巴胺(DA)的含量;化学比色法检测小鼠黑质(SN)部位超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)及单胺氧化酶B(MAO-B)。结果:与正常组比较,模型组小鼠爬杆时间显著延长(P<0.05),自主活动次数显著减少(P<0.05),纹状体DA含量、SOD及GSH-Px水平显著降低(P<0.01),MDA及MAO-B水平显著升高(P<0.01)。与模型组比较,经过BCT治疗,PD小鼠的神经行为学得到显著改善(P<0.05),纹状体DA含量,SOD及GSH-Px水平显著升高(P<0.01,P<0.05),MDA及MAO-B水平显著降低(P<0.01)。结论:BCT可以通过提高机体的抗氧化和清除自由基能力,明显改善PD小鼠的神经行为学变化及纹状体DA的含量,是其对PD模型神经保护作用的可能机制。Objective： To investigate the effect of Baichanting compound（ BCT） on neuroethology,oxidative stress response,and dopamine（ DA） content in mice with Parkinson＇s disease（ PD） induced by1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine（ MPTP）, and discuss the possible mechanism of BCT＇s neuroprotection effect in PD models. Method： Male C57 BL /6 mice were processed with MPTP-HCl（ 30 mg·kg^- 1·d^- 1,ip,0. 9% normal saline solution） for 5 days to establish PD mice models; the mice were randomly divided into normal group,model group,levodopa and benserazide tablets group（ 62. 50 mg·kg^- 1·d^- 1）,BCT high-dose,middle-dose and low-dose groups（ 363. 00,181. 50,95. 75 mg·kg^- 1·d^- 1）. All treatment groups were dissolved with 0. 5% carboxyl methyl cellulose（ CMC-Na）; BCT compound was ig given once daily for 20 days.The pole and independent activity experiments were done to observe the mice behavior change. The content of DA in corpus striatum was detected by UPLC-MS-MS method. Superoxide dismutase（ SOD）,glutathione-peroxidase（ GSH-Px）,monoamine oxidase-B（ MAO-B）,and malondialdehyde（ MDA） levels in midbrain substantia nigra tissues were detected by chemical colorimetry method. Result： Compared with the normal group,pole climbing time was significantly longer in model group（ P〈0. 05）,autonomic activity significantly reduced（ P〈0. 05）,content of DA in corpus striatum,SOD and GSH-Px levels significantly reduced（ P〈0. 01）,MDA and MAO-B levels significantly increased（ P〈0. 01）. Compared with the model group,neural behavior was improved significantly（ P〈0. 05）,the content of DA in corpus striatum,the activity of SOD and GSH-Px were increased significantly（ P〈0. 01,P〈0. 01）,and MDA and MAO-B levels were significantly reduced（ P〈0. 01） in PD model mice after the treatment of BCT. Conclusion： BCT can significantly improve neurobehavioral change and the content of striatal DA in PD mice by improving the body＇s ability to res

关 键 词：拜颤停 帕金森病 氧化应激 1-甲基-4-苯基-1 2 3 6-四氢吡啶 

分 类 号：R285.5[医药卫生—中药学]