肠外翻囊法研究淫羊藿肠吸收成分及其吸收特征  

Intestinal Absorption Ingredients in Epimedii Folium and Investigation of Their Absorption Characteristics by Everted Intestinal Sac Method

作　　者：秦海蛟[1] 柳梦婷 唐力英[2] 战皓 方婧[2] 杨洪军[2] 付梅红[2] 王斌[1] 吴宏伟[2] 谢练武[1] 

机构地区：[1]中南林业科技大学理学院,长沙410004 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2015年第22期163-168,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2011BAI07B08);中国博士后科学基金项目(2013M531787);湖南省科技计划项目(2013RS4035);人社部留学回归人员择优重点项目

摘　　要：目的:研究淫羊藿肠吸收成分及其吸收特性。方法:采用大鼠外翻肠囊模型,收集高、中、低质量浓度淫羊藿提取物给药后不同时间的肠囊液,采用HPLC检测肠吸收液样品中5种黄酮类成分的含量,计算累计吸收量和吸收速率常数,比较淫羊藿提取物与肠吸收液中指标成分的比例变化情况。结果:提取物中主要黄酮类成分均可在肠吸收液中检测到,针对其中5种主要成分朝藿定A,朝藿定B,朝藿定C,淫羊藿苷与淫羊藿次苷Ⅱ成功建立了定量分析方法。提取液中、低质量浓度时,这5种成分分别在空肠前、中段的累积吸收量最大,而高质量浓度则后移至回肠段;3种给药质量浓度下,5种成分的肠吸收均为线性吸收(R2>0.9),符合零级吸收速率;吸收速率常数随着给药浓度的增加而增大,提示各成分的体外肠吸收为被动运输;不同质量浓度淫羊藿肠吸收液的成分间比例与原提取物中各成分间比例存在差异。结论:淫羊藿中朝藿定A,朝藿定B,朝藿定C,淫羊藿次苷Ⅱ与淫羊藿苷5种成分均可被小肠吸收,外翻肠囊法可有效地评价各成分的吸收特征。Objective： To identify intestinal adsorption ingredients of Epimedii Folium and investigate their absorption characteristics in different intestinal segments. Method： Everted intestinal sac model was utilized.Intestinal sac fluid samples at different time after administration of high,medium and low concentration of Epimedii Folium extract were collected and five flavonoids in fluid samples were determined by HPLC. Accumulative absorbed doses（ Q） and absorption rate constants（ Ka） of five flavonoids were calculated,while proportions of different flavonoid between Epimedii Folium extract and intestinal absorption liquid were compared. Result：Epimedin A, epimedin B, epimedin C, icariin and icarisid Ⅱ were identified and could be quantitatively analyzed in extract and intestinal sac fluid samples of Epimedii Folium. Q of five flavonoids reached maximum value in front and middle jejunum segments under medium and low concentrations of Epimedii Folium extract,while Q of five flavonoids reached maximum value in ileum under high concentrations of extract. In these three concentrations of Epimedii Folium extract,absorption model of five flavonoids was linearity with R2〉 0. 9,which was in consistent with zero order absorption rate. Kaof five flavonoids increased with increasing of concentration of Epimedii Folium extract, which indicated that intestinal adsorption mechanism of five flavonoids was passive diffusion. Proportions of five flavonoids in intestinal sac fluid were different from those in Epimedii Folium extract.Conclusion： Epimedin A,epimedin B,epimedin C,icariin and icarisid Ⅱ in Epimedii Folium extract can be absorbed in whole intestine. Everted intestinal sac model can be used to evaluate intestinal absorption characteristics of ingredients in Epimedii Folium extract effectively.

关 键 词：淫羊藿 外翻肠囊法 朝藿定A 淫羊藿苷 淫羊藿次苷Ⅱ 

分 类 号：R945[医药卫生—微生物与生化药学] R969.1[医药卫生—药剂学]