治疗心血管疾病常用中药组分提取分离方法研究概况  

Research Survey of Extraction and Separation Technology of Effective Components in Common Chinese Medicines for Cardiovascular Disease

作　　者：王淳[1] 刘丽梅[1] 宋志前[1] 董运茁 杜智勇[1] 宁张弛 刘元艳[2] 刘振丽[1] 

机构地区：[1]中国中医科学院中医基础理论研究所,北京100700 [2]北京中医药大学中药学院,北京100102

出　　处：《中国实验方剂学杂志》2015年第22期169-175,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重大基础研究发展计划(973计划)项目(2012CB518401)

摘　　要：查阅中国知网、万方数据知识服务平台和Pub Med等数据库(截至2014年9月1日)及相关参考书目,对目前治疗心血管疾病最常用中药组分的提取分离方法相关文献进行汇总。研究显示最常用的提取方法有回流提取法、超声法、微波法、超临界萃取法、酶法和闪式提取法等,且研究多为实验室规模。提取工艺条件优化的方法主要有单因素试验、正交试验、均匀设计等。提取溶剂多为水和不同体积分数乙醇。最常用的分离纯化方法是大孔吸附树脂法,萃取法和沉淀法也常使用,并且常与大孔吸附树脂法联合应用以达到纯化目的。一些新的分离纯化技术比如超滤法、生物转化技术和分子印迹技术等也有使用,为治疗心血管疾病常用中药组分提取分离方法提供了系统的文献研究基础。We searched CNKI,Wanfang data,Pub Med and reference lists of identified articles（ As of September 1,2014）, and reviewed literature, summarized extraction and separation technology of effective components in the most common Chinese medicines for cardiovascular disease. The most common extraction methods were refluxing extraction,ultrasonic extraction,microwave extraction,supercritical extraction,enzyme method,flash extraction and so on,but these researches were usually for laboratory scale. Single factor design,orthogonal design, uniform design and etc were widely used for optimization of extraction method. Water and different concentration of ethanol were always used as extraction solutions. The most common separation methods were macroporous resin,solvent extraction and precipitation method,and the later two methods usually used with macroporous adsorption resin. Some new separation techniques, such as ultrafiltration method, biological transformation technology and molecular imprinting technique,were also used. This article can provide a literature basis for extraction and separation method of Chinese medicines in cardiovascular disease.

关 键 词：心血管疾病 中药组分 丹参 纯化工艺 文本挖掘 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]