刺五加与短梗五加种子的蛋白质电泳分析  

Electrophoresis Analysis of Protein in Seeds of Acanthopanax senticosus and A. sessiliflorus

作　　者：李晓琳[1] 张顺捷[2] 李颖[1] 孟黎明[2] 徐学良[1,3] 陈敏[1] 

机构地区：[1]中国中医科学院中药资源中心,道地药材国家重点实验室培育基地,北京100700 [2]黑龙江省林副特产研究所,黑龙江牡丹江157011 [3]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157012

出　　处：《中国实验方剂学杂志》2015年第22期180-183,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家“重大新药创制”科技重大专项(2012ZX09304006);国家自然科学基金项目(81102757)

摘　　要：目的:建立适用于刺五加和短梗五加种子的十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)鉴别方法,为二者种子的鉴别及质量评价提供参考。方法:采用15%分离胶的SDS-PAGE电泳对15个产地的刺五加种子和2个产地的短梗五加种子中可溶性蛋白进行电泳分析,利用Band Scan 5.0软件对蛋白谱带蛋数目、谱带强度和相对分子质量等方面的差异进行比较。结果:17个种子样品共分离出31条清晰的蛋白谱带,其中2种种子的10条共有特征谱带和各自的1条标准特征谱带可作为刺五加和短梗五加种子的标准蛋白图谱。不同产地的刺五加种子蛋白电泳图谱具有一定的种内差异性。结论:该电泳方法和标准蛋白图谱可作为刺五加及短梗五加种子的鉴别依据。Objective： To establish an appropriate sodium dodecyl sulfonate-polyacrylamide gel electrophoresis（ SDS-PAGE） method for identification of Acanthopanax senticosus and A. sessiliflorus seeds.Method： Using 15% gel SDS-PAGE,soluble proteins in A. senticosus seeds from 15 different locations and A.sessiliflorus seeds from 2 different locations were analyzed. Difference of protein band numbers,band intensity and protein molecular weight were compared by Band Scan 5. 0 software. Result： Total protein bands of 17 different seed samples were 31,10 common bands and their own specific band of two seeds could be standard protein fingerprints of A. senticosus and A. sessiliflorus seeds. There were some differences in protein bands among A.senticosus seeds from various locations. Conclusion： This electrophoresis method and the standards protein fingerprints can be applied to identify seeds of A. senticosus and A. sessiliflorus.

关 键 词：刺五加 短梗五加 蛋白质 十二烷基磺酸钠-聚丙烯酰胺凝胶电泳 电泳图谱 

分 类 号：R282.5[医药卫生—中药学] R284.1[医药卫生—中医学]