颈动脉粥样硬化患者外周血中CD36表达水平及与Toll样受体4信号的相关性  

Expression of CD36 in peripheral blood of atherosclerosis patients and its relation to TLR4 signaling

作　　者：杜冉[1] 张会丽[1] 赵莘瑜[1] 牛瑞娜[1] 

机构地区：[1]郑州大学第一附属医院,河南郑州450052

出　　处：《中国现代医学杂志》2015年第32期31-35,共5页China Journal of Modern Medicine

摘　　要：目的观察颈动脉粥样硬化(AS)患者外周血中CD36水平,并探讨其与人单核细胞Toll样受体4(TLR4)信号的相关性。方法选取该院治疗的颈AS患者40例为AS组,以同期来该院体检的40例健康者为对照组。收集两组患者外周血后采用逆转录-聚合酶链反应(RT-PCR)及Western blot检测CD36水平和TLR4信号激活的相关因子。采用匹伐他汀抑制AS组患者单核细胞中的CD36水平后,检测AS组患者单核细胞中相关因子的表达。结果与对照组比较,AS组患者外周血中CD36的m RNA及蛋白水平显著提高,差异有统计学意义(t=41.503和27.112,P=0.000)。AS组患者外周血中肿瘤坏死因子-α(TNF-α)为(50.2±3.1)pg/ml,显著高于对照组[(33.5±2.8)pg/ml];AS组患者外周血中白介素-6(IL-6)为(54.1±2.4)pg/ml,显著高于对照组[(32.8±2.1)pg/ml],差异有统计学意义(t=42.242,P=0.000);AS组患者外周血中核转录因子(NF-κB)m RNA水平显著高于对照人群,差异有统计学意义(t=34.411,P=0.000)。CD36 m RNA水平与患者血清TNF-α、IL-6及NF-κB m RNA呈正相关,差异有统计学意义(r=0.641、0.579和0.659,P<0.05)。采用匹伐他汀抑制AS组患者体内CD36表达后,AS组患者外周血中TNF-α和IL-6蛋白及NF-κB m RNA水平显著低于治疗前,差异有统计学意义(t=17.277、10.022和15.360,P=0.000)。结论 AS患者外周血中CD36高表达,并与TLR4信号相关转录因子及细胞因子水平相关,提示CD36可能通过影响TLR4信号参与AS的发生、发展。[Objective] To observe the levels of CD36 in peripheral blood of atherosclerosis （AS） patients, and explore the relationship between CD36 and toll-like receptor （TLR4） signaling in atherosclerosis. [Methods] The study group included 40 cases of atherosclerosis patients in our hospital and the control group included 40 healthy volunteers. RT-PCR was used to detect the expression of CD36, and Western blot used to detect the protein levels related to TLR4 signal activation. In addition, Pitavastatin was used to inhibit the expression of CD36 in the AS patients, and then the TLR4 signal activation related genes and proteins were detected. [Results] Compared with the control group, the levels of CD36 mRNA and protein in the AS patients were significantly increased, the differences were statistically significant （t=41.503 and 27.112, P= 0.000）. The serum TNF-ct level in the peripheral blood of the AS patients was （50.2 ± 3.1）pg/ml which was significantly higher than （33.5± 2.8） pg/ml in the control group （t = 25.284, P= 0.000）. The serum IL-6 level in the patients with AS was （54.1 ± 2.4） pg/ml which was significantly higher than （32.8±2.1）pg/ml in the control group （t = 42.242, P= 0.000）. The level of NF-KB mRNA in the peripheral blood of the AS patients was significantly higher than that of the control group （t = 34.411, P= 0.000）. In addition, there were positive correlations between CD36 mRNA level and TNF-α, IL-6 and NF-KB mRNA levels （r= 0.641, 0.579 and 0.659, P〈 0.05）. After using Pitavastatin to inhibit CD36 expression in the AS patients, the serum levels of TNF-α, IL-6 and NF-KB mRNA were significantly lower than those before inhibition （t = 17.277, 10.022 and 15.360; P=0.000）. [Conclusions] CD36 is highly expressed in patients with AS, and CD36 expression is significantly associated with TLR4 signal related transcription factors and eytokine levels in AS patients, sug- gesting that CD36 may be involved in the occurrence and development of AS by affectin

关 键 词：动脉粥样硬化 CD36 TOLL样受体4 

分 类 号：R543.4[医药卫生—心血管疾病]