静脉输注丙种球蛋白后对交叉配血的影响  

Effect of intravenous immunoglobulin on cross matching

作　　者：刘素芳[1] 张烨[1] 张磊[1] 刘凯[1] 许志远[1] 刘亚庆[1] 

机构地区：[1]北京市红十字血液中心血型室,100088

出　　处：《北京医学》2015年第11期1114-1116,共3页Beijing Medical Journal

摘　　要：目的研究静脉输注丙种球蛋白后对患者交叉配血的影响。方法对2014年医院送检的8例抗体筛选阴性、交叉配血不合的患者标本,采用试管法检测血型,采用微柱凝胶技术做抗体筛选、交叉配血、直接抗人球蛋白试验,检测血浆中抗-A、抗-B,热放散试验,用Liss/Coombs卡检测放散液。结果患者静脉输注丙种球蛋白后,5例A型和2例AB型患者血浆中检测到抗-A,1例B型患者血浆中检测出抗-B,患者直接抗人球蛋白试验阳性,且都与同型悬浮红细胞交叉配血不合。结论静脉丙种球蛋白制剂中含有的ABO血型抗体可引起交叉配血不合、血型鉴定困难。Objective To investigate the effect of intravenous immunoglobulin（IVIG） on cross matching. Methods The blood samples of 8 patients whose antibody screening were negative and cross matching were imcompatibale from the hospitals in 2014 were tested. Tube test method was used for ABO typing; microcolumn gel technique was used for antibody screening, cross matching, direct antiglobulin test and anti-A／B in serum and LISS/Coombs method was used for detecting elution. Results Among 8 cases after IVIG infusion, anti-A was detected in 5 cases A and 2 cases for AB type, anti- B was detected in 1 case. DAT of all cases was positive and cross- matching was incompatibale. Conclusion The ABO antibodies contained within IVIG can cause cross-matching incompatibility and ABO discrepancy.

关 键 词：静脉注射用人血丙种球蛋白 交叉配血 热放散试验 直接抗人球蛋白实验 间接抗人球蛋白实验 

分 类 号：R446.6[医药卫生—诊断学]