光学相干断层显像在基于罗叶泵驱动下组织工程血管构建中的应用  

Application of optical coherence tomography in tissue engineered blood vessel culture based on Luo-Ye pump

作　　者：陈婉雯[1] 廖文君[2] 吴岳恒[4] 李东风[4] 周嘉辉[3] 杨峻青[3] 林展翼[5] 

机构地区：[1]南方医科大学心内科广东省人民医院心内科广东省医学科学院,510515 [2]南方医科大学广东省第二人民医院心内科 [3]广东省人民医院广东省医学科学院广东省心血管病研究所心内科 [4]南方医科大学广东省第二人民医院医学研究中心 [5]广东省老年医学研究所

出　　处：《中华胸心血管外科杂志》2015年第11期687-690,共4页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：广东省科技计划项目（20098060700116,20138010404034）

摘　　要：目的利用光学相干断层显像技术（OCT）对三维培养的组织工程血管（TEBV）进行动态监测，探讨其在组织工程血管构建中的应用价值。方法组织块法原代培养获得脐动脉平滑肌细胞，经传代培养后接种到聚乙醇酸（PGA）支架上，在以左心循环辅助罗叶泵提供脉动式张应力的三维培养系统上进行培养。于培养第1、4、7、10、14、17、21天应用OCT进行监测并获取影像学资料。培养结束时，结合组织学染色分析OCT对TEBV的成像能力。结果OCT清晰显示PGA逐渐降解时支架复合物厚度逐渐降低，组织结构从疏松到致密，培养第21天见血管表面光滑平整且细胞外基质分布均匀。结合组织学染色显示培养血管结构与正常血管接近。培养结束时OCT获得TEBV厚度与组织学比较存在良好相关性（r=0．922，P〈0．05）。结论OCT能对基于罗叶泵为驱动装置的三维培养系统下构建的TEBV微结构进行清晰成像，并可显示组织在生物反应器内的血管重构化过程，为TEBV的构建提供一种有效方便的动态监测方法。Objective To explore the application of optical coherence tomography in vascular tissue engineering culture by dynamic monitoring its changes. Methods Human umbilical artery smooth muscle cells were isolated and culture by tissue block method. After passage culture and cell surface markers evaluation, smooth muscle cells were seeded onto polyglycolic acid scaffold and placed into the bioreactor based on Luo-Ye pump with pulsatile stress for three-dimensional culture. At 1,4, 7,10,14,17,21 days in culture, the image data was obtained by optical coherence tomography technology. The ability of ima- ging TEBV via OCT was analyzed combined with histopathological observation. Results As the incubation time extended, OCT clearly showed PGA gradual degradation, decreased composite scaffold thickness and the wall structure from loose to tight. At 21 days in culture, the vessel mimics had smooth surface with extracellular matrix evenly distributed and achieved complete reconstruction in the PGA scaffold. Combining with histopathological staining, the blood vessel mimics were similar to natural blood vessels. OCT measured TEBV thickness compared with histopathological measurement had good correlation （ r = 0. 922, P 〈 0.05 ）. Conclusion Optical coherence tomography could clearly image mierostructures of tissue engineered blood vessels cultured in three-dimensional culture system based on Luo-Ye pump, delineate the reconstruction of TEBV-like tissue in the bioreactor and provide as a dynamic and convenient monitoring tool in vascular tissue engineering.

关 键 词：光学相干断层显像 组织工程 血管 培养 

分 类 号：R318.11[医药卫生—生物医学工程]