集成微流控芯片在单细胞测序样品制备中的应用  

Sample preparation for single cell sequencing on integrated microfluidic devices

作　　者：虞之龙[1,2] 黄岩谊[1,2,3,4] 

机构地区：[1]北京大学生物动态光学成像中心,北京100871 [2]北京大学工学院,北京100871 [3]北京大学-清华大学生命科学联合中心,北京100871 [4]北京大学化学与分子工程学院,北京100871

出　　处：《中国科学：化学》2015年第11期1090-1101,共12页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(91313302)资助

摘　　要：近年来,单细胞测序技术的飞速发展,从更精细的水平上揭示了细胞在遗传物质上的异质性.微流控技术作为操纵微量液体的新兴技术,尤其适合单细胞这样的微观物体操纵.本文着重介绍了集成微流控芯片在单细胞测序样品制备领域中的应用,包括单细胞全基因组测序样品制备、单细胞转录组测序样品制备和少量细胞的表观遗传组测序样品制备.相比传统方法,集成微流控芯片不仅在单细胞的操纵和俘获上有着独特的优势,也更容易进行高通量的并行实验,结果也显示了高度的平行性和重复性.集成化微流芯片在单细胞测序样品制备领域显示出了巨大的应用前景.Recently, single cell sequencing has become the cutting-edge technology to reveal the complexity of biological processes at the finest scale. However, it is not easy to handle single cells precisely, nor to perform the small scale reactions accurately. Microfluidics offers a ready solution to control the liquid delivery at the picoliter scale, matching the accuracy requirements for single cell analyses. We review the recently reported applications that using integrated microfluidic devices to facilitate the sample preparation for single cell sequencing. Various sequencing approaches with very limited starting materials, such as single cell whole-genome sequencing, single cell whole-transcriptome sequencing, and 1,000-cell chromatin immunoprecipitation sequencing, have been successfully performed with microfluidic assistance. Compared to conventional methods, integrated microfluidic devices exhibit unique advantages in manipulation and capturing single cells, and provide flexible tools for performing high throughput experiments in parallel. The results are highly repeatable and reproducible, showing great potential in sample preparation for single cell sequencing.

关 键 词：微流控 集成化 单细胞测序 样品制备 

分 类 号：TN492[电子电信—微电子学与固体电子学]