阴离子交换色谱在DNA纳米结构纯化中的应用  

Purification of DNA nanostructures by anion exchange chromatography

作　　者：邢淑[1] 江大卫[1] 崔呈俊[1] 王丽华[1] 黄庆[1] 

机构地区：[1]中国科学院界面物理与技术重点实验室,上海同步辐射光源,中国科学院上海应用物理研究所物理生物学实验室与生物成像中心,上海201800

出　　处：《中国科学：化学》2015年第11期1220-1225,共6页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(31371015,21329501);国家重点基础研究发展计划(2012CB932603)资助

摘　　要：随着DNA纳米技术的发展,研究快速、简便、有效的纯化方法,提高DNA纳米结构的产率、纯度和规模等成为人们关注的热点.本文使用阴离子交换色谱（AEC）技术纯化了5种不同棱长（13、17、20、26和30 bp）的DNA四面体及一种棱长（20 bp）的DNA三角双锥结构,将这些DNA纳米结构从初始合成纯度30%~65%提高到95%以上.利用纯化后的带有互补手臂链的两个四面体杂交构建哑铃状DNA纳米结构,其产率从11%提高到90%.AEC纯化解决了DNA纳米结构应用中存在的纯度、产率等瓶颈问题,为其在生物学和医学领域中的应用奠定了基础.A purification step after synthesis has become a standard procedure for many DNA nanostructures studies. We firstly applied anion exchange chromatography（AEC） to DNA nanocages purification. A 20 bp-edge DNA bipyramid and five DNA tetrahedra with different edge lengths including 13, 17, 20, 26 and 30 bp were purified effectively by AEC, which improved the purity of these nanostructures from 30%–65% up to 95%. And the yield of a dumbbell-like nanostructure which was constructed using AEC-purified DNA tetrahedra was more than 90%, but 11% for the unpurified group. Given the versatility of this method, AEC is promising for other DNA nanocages and other DNA nanostructures purification.

关 键 词：阴离子交换色谱 分离纯化 DNA纳米结构 哑铃状结构 

分 类 号：O629.74[理学—有机化学] TB383.1[理学—化学]