Ghrelin对吗啡肠麻痹小鼠结肠动力的影响  

Effect of ghrelin on the colonic motility of morphine induced bowel dysfunction of mice

作　　者：赵晶[1] 汪艳[1] 许涛[1] 江伟[1] 

机构地区：[1]上海交通大学附属第六人民医院麻醉科,上海200233

出　　处：《上海交通大学学报（医学版）》2015年第11期1687-1690,共4页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的探讨生长激素促分泌素受体内源性激动剂ghrelin对吗啡肠麻痹小鼠结肠动力的影响。方法C57小鼠50只,分别进行在体酚红推进实验和离体结肠肌条收缩实验。在体实验中,25只小鼠均分为5组,一组作为正常组,另4组建立吗啡肠麻痹模型后分别腹腔注射生理盐水和不同剂量的ghrelin(50、100和200μg/kg)。酚红推进实验计算各组小鼠结肠推进率。离体实验中,25只小鼠亦均分为5组,分别为正常组、吗啡组和3个ghrelin治疗组(ghrelin浓度分别为0.1、1和10μmol/L);取小鼠结肠段,用生物信号处理系统测定各组结肠肌条自发收缩幅度。结果在体实验中,吗啡肠麻痹小鼠的结肠推进率为(33.77±0.49)%,注射ghrelin 50、100和200μg/kg后,结肠推进率分别为(41.42±1.66)%、(45.86±0.86)%和(56.84±0.84)%,均显著高于吗啡肠麻痹未治疗小鼠(P<0.05)。离体实验中,吗啡组小鼠的结肠肌条收缩幅度为(0.568±0.014)g;ghrelin浓度为0.1、1和10μmol/L时,收缩幅度分别增至(0.624±0.118)、(0.673±0.014)和(0.712±0.125)g,均显著高于吗啡组(P<0.05)。结论 Ghrelin可以显著提高吗啡肠麻痹小鼠的结肠动力。Objective To investigate the effect of growth hormone secretagogue receptor agonist ghrelin on the colon motility of morphine induced bowel dysfunction of mice.Methods Phenolsulfonphthalein pushing test in vivo and contraction test of colonic muscle strips in vitro were conducted for 50 C57 mice.For in vivo test,25 mice were divided into five groups.One group was the control group and mice of other four groups were intraperitoneally injected with saline or different doses（50,100,and 200 μg/kg） of ghrelin after the model of morphine induced intestinal paralysis was established.The colonic transit of mice was measured by phenolsulfonphthalein pushing test.For in vitro test,25 mice were also divided into 5 groups,i.e.the control group,morphine group,and three ghrelin groups（ghrelin of 0.1 μmol/L,1 μmol/L,and 10 μmol/L）.Mouse colons were harvested and the spontaneous contraction amplitude of colonic muscle strips was recorded by the biological signal system.Results For in vivo test,colonic propulsive rates of the morphine group were（33.77±0.49）%.After being injected with ghrelin of 50,100,and 200 μg/kg,colonic propulsive rates were（41.42 ±1.66）%,（45.86± 0.86）%,and（56.84 ±0.84）%,respectively,which were significantly higher than those of the morphine group（P〈0.05）.For in vitro test,contractions amplitudes of colon muscle strips of the morphine group were（0.568±0.014） g.After being treated with ghrelin of 0.1 μmol/L,1 μmol/L,and10 μmol/L,contraction amplitudes increased to（0.624 ±0.118）,（0.673±0.014）,and（0.712 ±0.125） g,respectively,which were significantly higher than those of the morphine group（P 〈0.05）.Conclusion Ghrelin can significantly improve the colonic motility of morphine induced bowel dysfunction of mice.

关 键 词：生长激素促分泌素受体激动剂 吗啡肠麻痹 结肠动力 

分 类 号：R614[医药卫生—麻醉学]