eNOS基因多态性与糖尿病视网膜病变的相关性研究  

Association between endothelial nitric oxide sythasea gene polymorphism and diabetic retinopathy

作　　者：董娟[1] 孟宇[2] 

机构地区：[1]皖南医学院机能学实验中心,241000 [2]皖南医学院生物化学教研室,241000

出　　处：《齐齐哈尔医学院学报》2015年第30期4530-4532,共3页Journal of Qiqihar Medical University

基　　金：安徽省自然科学基金项目(1308085QH134)

摘　　要：目的探讨内皮细胞型一氧化氮合酶(e NOS)基因第4内含子4a/b多态性与糖尿病视网膜病变的关系。方法 e NOS基因第4内含子4a/b多态性采用聚合酶链反应扩增片段长度多态性(PCRAFLP)技术,结果 e NOS基因4a/b多态性与糖尿病视网膜病变的关系:正常组、DM、DR三组b、a等位基因频率相比差异无统计学意义(χ2=1.260,P>0.05)。三组bb、ab、aa基因型频率相比差异无统计学意义(χ2=0.326,P>0.05)。三种基因型在引起糖尿病视网膜患者的发病风险上无明显差异。结论 e NOS基因第四内含子4a/b多态性与糖尿病视网膜病变未见有关联。Objective To investigate the association between a 4a/b polymorphism at intron 4 of e NOS gene and diabetic retinopathy. Methods 4a / b polymorphism at intron 4 of e NOS was identified with a PCRAFLP. Results Association between the 4a / b polymorphism at intron 4 of e NOS and diabetic retinopathy： There＇s no significant deviation between Control,DM,DR groups at b and a allele frequencies（ χ2= 1. 260,P〈0. 05）.The bb,ab,aa genotypic frequencies of cases in the three groups had no significant deviation（ χ2= 0. 326,P〈0. 05）. The 4a / b polymorphism at intron 4 of e NOS was not associated with the susceptibility of DR.Conclusions The 4a / b polymorphism at intron 4 of e NOS is not associated with the susceptibility of DR.

关 键 词：内皮细胞型一氧化氮合酶 基因多态性 糖尿病 糖尿病视网膜病变 

分 类 号：R587.2[医药卫生—内分泌]