主动外排机制和膜蛋白缺失在粘质沙雷菌耐药中的研究  

Study on the active efflux mechanism and the absence of membrane proteins in Serratia marcescens resistence

作　　者：李欣 汪建军 任超杰 扈会整 王欣 

机构地区：[1]西电集团医院,西安710077 [2]陕西省核工业215医院

出　　处：《陕西医学杂志》2015年第11期1510-1512,共3页Shaanxi Medical Journal

基　　金：陕西省科学技术研究发展计划项目(2014K11-02-03-09)

摘　　要：目的:研究粘质沙雷菌的耐药性与细胞的主动外排机制及细菌膜蛋白缺失相关性。方法:收集对亚胺培南和美罗培南耐药的粘质沙雷菌株15株,应用PCR扩增和DNA序列分析,采用E试验观察氰氯苯腙对亚胺培南最低抑菌浓度(MIC)的影响,以研究细胞内是否存在主动外排机制和膜蛋白缺失现象。结果:当CCCP存在时,其中7株粘质沙雷菌亚胺培南,美罗培南的MIC值下降,提示存在主动外排机制。其中有1株OprD2蛋白缺失,1株外排泵MexB基因阳性。结论:耐碳青霉烯类药物的粘质沙雷菌的耐药性与细胞的主动外排机制和膜蛋白缺失有关。Objective：Study the drug resistance of Serratia marcescens whether related to the cells active efflux mechanism and the absence of bacterial membrane proteins or not.Methods：collected 15 strains Serratia marcescens that resist Imipenem and Meropenem,application PCR amplification and DNA sequence analysis.adopt E test experiment observe the influence of CCCP for Imipenem minimal inhibitory concentration.in order to study intracellular was exist active efflux mechanism and the absence of membrane proteins or not.Results：When CCCP exist,there were 7strains serratia marcescens for imipenem and Meropenem＇MIC value decreased,prompting exist active efflux mechanism bacterial,and 1strain absent OprD2 protein,1strain efflux pump MexB gene positive.Conclusion：The resistance of Serratia marcescens that resist Carbapenemase related to the cells active efflux mechanism and the absence of bacterial membrane proteins.

关 键 词：粘质沙雷菌 亚胺培南 @MexB基因 @OprD2蛋白 抗药性 细菌 

分 类 号：R446.5[医药卫生—诊断学]