PCR-RDB技术在HCV基因分型中的应用研究  

Application research of genotyping for hepatitis C virus by PCR-RDB

作　　者：李步荣[1] 张彤[1] 李丽华[1] 高宁[1] 张毅[1] 

机构地区：[1]西安交通大学第二附属医院检验科,西安710004

出　　处：《陕西医学杂志》2015年第11期1551-1553,共3页Shaanxi Medical Journal

摘　　要：目的:评价PCR-RDB技术在丙型肝炎病毒(HCV)基因型检测中的临床实际应用价值。方法:随机收集经实时荧光定量聚合酶链式反应(PCR)检测HCV RNA含量大于1×103 IU/ml血清样本总计158份,其中无临床症状丙型肝炎病毒携带者90例,慢性丙型肝炎(CHC)患者48例,肝硬化(HS)患者16例,肝癌(LC)患者4例。全部血清样本应用PCR和反向斑点杂交技术(RDB)进行HCV基因分型检测,并对检测结果进行统计学分析。结果:所有标本HCV基因分型成功,其中1b型78例,2a型65份,3a型3例,3b型2例,6a型2例,1b和2a混合感染型8例。不同疾病组HCV不同基因型分布差异无统计学意义。结论:HCV基因型与疾病类型无相关性;PCR-RDB杂交技术适用于临床实验室开展HCV基因型检测。Objective：To evaluate the clinical practical application value of polymerase chain reaction and reverse dot blot（PCR-RDB）method on genotyping HCV in different disease groups.Methods：We conducted a cohort study with 158 participants including 90 HCV carriers without clinical symptom,48 patients with chronic hepatitis C,16 patients with hepatic sclerosis and 5patients with liver cancer.The HCV RNA levels of all sera samples were confirmed higher than 1×103 IU/ml by real-time fluorescent quantitative polymerase chain reaction（PCR）.HCV genotyping was carried out using type-specific primers by means of polymerase chain reaction and reverse dot blot（RDB）.And the results were statistically analyzed with GraphPad Prism 5.0software.Results：All 158 specimens were genotyped successfully.Among them,78 cases were genotype 1b,65 cases were genotype 2a,3cases were genotype 3a,2cases were genotype 3b,2cases were genotype 6a,8cases were mixed genotypes 1band 2a.There was no significant difference among the different disease groups about the distribution of HCV genotypes.Conclusion：There is no relationship between the HCV genotypes and disease types.PCR-RDB was a suitable method for the HCV genotyping in clinical laboratories.

关 键 词：丙型肝炎病毒 基因分型技术 聚合酶链式反应 荧光免疫测定 

分 类 号：R446.6[医药卫生—诊断学]