新疆伊犁州维吾尔族20个STR基因座的遗传多态性  

The genetic polymorphism of 20 STR loci in Uygur population of Yili of Xinjiang

作　　者：高波 邱平明[2] 

机构地区：[1]新疆伊犁州奎屯市公安局,新疆伊犁833200 [2]南方医科大学法医学系,广东广州510515

出　　处：《分子诊断与治疗杂志》2015年第6期407-411,共5页Journal of Molecular Diagnostics and Therapy

摘　　要：目的调查新疆伊犁州地区维吾尔族人群20个STR基因座（D1S1656,D2S1338,D3S1358,D5S818,D6S1043,D7S820, D8S1179,D12S391,D13S317,D16S539, D18S51,D19S433,D21S11, Amelogenin,CSF1PO,FGA,Penta D,Penta E,TH01,TPOX和v WA）的遗传多态性及法医学应用参数。方法应用Promega公司Power Plex21试剂盒对300名维吾尔族无相关个体血样进行扩增,3500XL遗传分析仪对扩增产物进行检测,Gene Mapper ID-X软件进行结果分析,计算基因座的基因频率以及群体遗传学参数。结果在300名无相关维吾尔族个体中,共发现221个等位基因,其中单基因频率在0.0004~0.527 1之间,杂合度（heterozygosity,H）值在0.679 4~0.882 4,个体识别力（probability of discrimination power,DP）值在0.793 8~0.980 6,匹配率（match probability,Pm）值在0.031 2~0.104 2,非父排除率（probability of exclusion,PE）值在0.401 2~0.759 5,多态信息含量（polymorphism information content,PIC）值在0.593 8~0.910 5。结论 20个STR基因座在新疆伊犁州地区维吾尔人群具有高度的遗传多态性,满足该群体法医学个体识别及亲权鉴定的需要。Objective To investigate the genetic polymorphisms of 20 STR loci（D1S1656, D2S1338,D3S1358, D5S818, D6S1043, D7S820, D8S1179, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11,Amelogenin, CSF1 PO, FGA, Penta D, Penta E, TH01, TPOX and v WA） in unrelated Uygur individuals in Yili of Xinjiang and to explore the application value in forensic practice. Methods Blood samples from 300 unrelated Uygur individuals were amplified by using Power Plex21 kit. The amplified products were detected by using 3500 XL Genetic Analyzer and the genotyping was done by using Gene Mapper ID-X. Allele frequency of all loci and population genetics parameters were calculated. Results Of the 300 unrelated Uygur individuals,221 alleles were detected, with an allele frequency of 0.000 4~0.527 1. The heterozygosity（H） values were0.679 4~0.882 4, probability of discrimination power（DP） values were 0.793 8~0.980 6, match probability（Pm）values were 0.031 2~0.104 2, probability of exclusion（PE） values were 0.401 2~0.759 5, polymorphism information content（PIC） values were 0.593 8~0.910 5. Conclusion The 20 STR loci in the Uygur population of Yili of Xinjiang have high genetic polymorphisms, and can be satisfied as genetic markers for population individual identification and paternity testing.

关 键 词：法医遗传学 多态性 短串联重复序列(STR) 维吾尔族 

分 类 号：D919.1[医药卫生—法医学]