基于响应面分析法对烟叶中果胶测定方法的优化  

The Optimization of the Method of Tobacco Pectin Determination Based on the Response Surface Methodology

作　　者：白晓莉 侯英 张朗 潘纯祥 陶自伟 刘静 杨蕾[2,3] 

机构地区：[1]云南中烟工业有限责任公司技术中心,昆明650202 [2]云南同创检测技术股份有限公司检测事业部,昆明650106 [3]昆明理工大学环境科学与工程学院,昆明650500

出　　处：《中国烟草科学》2015年第5期85-89,共5页Chinese Tobacco Science

基　　金：中国烟草总公司科技重大专项项目"再造烟叶工业应用的特色工艺研究"[110201301019(ZZ-08)];云南中烟工业有限责任公司技术中心项目"造纸法再造烟叶大分子物理处理技术研究"(JSZX2014JC14)

摘　　要：为了对酶解-连续流动法测定烟叶中果胶含量的方法进行优化,采用Box-Benhnken 实验设计及响应面分析法对酶解过程中的酶解温度、酶解时间和缓冲液pH 值进行优化.结果显示,酶解温度对果胶测定影响最大,缓冲液pH 次之,酶解时间影响最小,且最优酶解条件为酶解时间1.9 h、酶解温度56 ℃、缓冲液pH 3.8;在此条件下,果胶具有良好的线性关系（R2=0.999）,加标回收率在99.2%-100.9%,相对标准偏差RSD（n=6）〈3%;说明Box-Behnken 设计结合响应面分析法优化所得的果胶测定方法具有较好的精密度及准确度,适用于烟叶中果胶含量的批量测定.In order to optimize the method of determining tobacco pectin using enzymolysis-flow method, reaction temperature, enzymatic hydrolysis time and buffer p H were studied through the Box-Benhnken experimental design and response surface methodology. The results showed that reaction temperature had the greatest impact on the determination of pectin, followed by buffer p H, and the enzymatic hydrolysis time comes last. The optimal enzymatic hydrolysis conditions were p H 3.8, time 1.9 h and 56 ℃. Under this condition, the linear relationship of pectin was optimal(R2=0.999), with the recovery of pectin in tobacco ranged from 99.2% to 100.9%, and the RSD(n=6) was less than 3%. This suggested that the optimized enzymatic hydrolysis conditions used by the Box-behnken design and response surface analysis have good precision and accuracy and are suitable for testing large number of samples.

关 键 词：响应面分析 连续流动法 烟叶果胶 定量测定 

分 类 号：TS411[农业科学—烟草工业]