臭氧局部注射对急性软组织损伤的影响  

EFFECT OF OZONE LOCALLY INJECTED INTO RAT SKELETAL MUSCLE AFTER ACUTE CONTUSION

作　　者：贾雅蕊[1] 刘金锋[2] 

机构地区：[1]山东省菏泽市立医院麻醉科,菏泽274000 [2]哈尔滨医科大学附属第二医院疼痛科,黑龙江麻醉与危重病重点实验室,哈尔滨150086

出　　处：《中国疼痛医学杂志》2015年第11期834-839,共6页Chinese Journal of Pain Medicine

摘　　要：目的:探讨臭氧局部注射对急性软组织损伤大鼠血清细胞因子的影响。方法:将144只SD大鼠随机分为正常组(normal group,A组)、模型组(model group,B组)、空气组(air group,C组)、低浓度组(low concentration group,D组)20μg/ml臭氧、中浓度组(middle concentration group,E组)40μg/ml臭氧和高浓度组(high concentration group,F组)60μg/ml臭氧。除正常组,其他各组均建立大鼠急性软组织损伤模型,对比观察不同浓度的臭氧治疗急性软组织损伤的情况及血清中PGE2(prostaglandin E2)、TNF-α(Tumor Necrosis Factor alpha)和IL-6(interleukin-6)含量的变化。结果:与A组比较,自损伤开始其他五组TNF-α、IL-6和PGE2含量均显著升高(P<0.05)。与B组相比,从注射后第1天起D组和E组的TNF-α、IL-6及PGE2均显著下降,并在第14天接近A组。与B组相比,E组从注射臭氧后损伤症侯指数显著下降(P<0.05)。在治疗第14天时损伤组与其他各组的损伤症侯指数均无显著差异。结论:40μg/ml臭氧注射治疗能够明显的抑制创伤产生的无菌性炎症反应,促进损伤修复的作用。Objective： To investigate the ozone effects on cytokines of rat gastrocnemius after acute soft tissue injury. Methods： After the establishment of acute soft tissue injury model, rats were randomly divided into six groups： normal group; model group; air group; low concentration group（20μg/ml ozone）; middle concentration group（40μg/ml ozone）; and high concentration group（60μg/ml ozone）. The degree of injury in all groups and the content change of PGE2, IL-6 and TNF-α with treatment of different concentrations of ozone were observed. Results： Compared with group A, the level of PGE2, TNF-α and IL-6 increased significantly in the other five groups since tissue injury（P〈0.05）. Compared with group B, PGE2, IL-6 and TNF-α in group D and group E decreased significantly, and appeared close to group A on the fourteenth day. Compared with group B, symptom index of group D and E decreased significant（P〈0.05）. Compared with group B, all other groups had no significant difference on 14 th day. Conclusion： Local injection of 40μg/ml ozone can obviously inhibit the traumatic aseptic inflammation and promote the repair of tissue damage.

关 键 词：臭氧 急性软组织损伤 细胞因子 

分 类 号：R965[医药卫生—药理学]