葛根芩连汤对牙周炎大鼠血清hs-CRP、IL-6表达的影响  

The effects of Gegen Qinlian decoction on high sensitive C-reactive protein and interleukin-6 in serum of the rats with periodontitis

作　　者：罗欣[1] 王丹[1] 彭伟[1] 冯晓洁[1] 

机构地区：[1]河北联合大学口腔医学院牙周科,唐山063000

出　　处：《实用口腔医学杂志》2015年第6期780-783,共4页Journal of Practical Stomatology

摘　　要：目的:检测葛根芩连汤治疗前后牙周炎模型大鼠血清中超敏C-6应蛋白(hs-CRP)、白介素-6(IL-6)及牙周组织中IL-6的表达水平。方法:45只Wistar大鼠随机分成2组:正常对照N组(n=10),牙周炎模型P组(n=35)。模型验证(n=5)后P组随机分成3组(n=10)进行药物灌胃4周:P_0盐水组,P_1甲硝唑组,P_2葛根芩连汤组。给药结束时处死大鼠,即刻取血,ELISA法检测血清中hs-CRP和IL-6的水平。取大鼠实验牙牙周组织检测牙周附着丧失(AL)水平,免疫组化S-P法分析牙周组织中IL-6的表达。结果:P_1,P_2组牙周炎症状表现较P_0组有改善,P_2组改善明显。P_2组AL水平显著低于其他组(P<0.05)。P_2组牙周组织中IL-6的表达水平及血清hs-CRP,IL-6水平较其他组明显降低(P<0.05)。各组血清hs-CRP和IL-6的表达呈正相关。结论:葛根芩连汤通过降低血清中hs-CRP和IL-6的表达,改善牙周炎症状况。Objective：To observe the effect of Gegen Qinlian decoction on high sensitive C-reactive protein（hs-CRP） and interleukin-6（IL-6） in serum of the rats with periodontitis and the IL-6 expression level in periodontal tissues.Methods：45 Wistar rats were randomly divided into 2 groups：control group N（n = 10）,periodontitis model group P（n = 35）.After periodontitis model was identified by the examination of 5 rats,the rest rats were randomly divided into 3 groups（n = 10） and treated by gavage of saline（group P0）,metronidazole（group P1） and Gegen Qinlian decoction（group P2） respectively for 4 weeks.Then all the rats were sacrificed.Serum was immediately harvested for the test of serum hs-CRP and IL-6 levels by ELISA.Attachment loss level（AL） was examined by pathology.IL-6 expression in periodontal tissues was examined by S-P immunohistochemistry.Results：The inflammation of periodontal tissues in P1 P2 were improved more than that in P0.AL in group P2 were lower than that in other groups（P〈0.05）.The IL-6 expression in periodontal tissues and the levels of serum hs-CRP and IL-6 of group P2 were lower than those of other groups（P〈0.05）.Serum hs-CRP level was positively correlated with IL-6 level.Conclusion：Gegen Qinlian decoction may inhibit the development of peri odontitis by depressing the expression of serum hs-CRP and IL-6.

关 键 词：葛根芩连汤 牙周炎 超敏C-反应蛋白(hs-CRP) 白细胞介素-6(IL-6) 

分 类 号：R285.5[医药卫生—中药学]