Trichostatin A能导致小麦基因组DNA改变及甲基化修饰  

Trichostatin A Can Induce Wheat Genomic DNA Change and Methylation Modification

作　　者：赵峥[1] 黄豫谦[1] 张飞雄[1] 

机构地区：[1]首都师范大学生命科学学院,北京100048

出　　处：《首都师范大学学报（自然科学版）》2015年第6期60-64,共5页Journal of Capital Normal University：Natural Science Edition

摘　　要：为了研究组蛋白去乙酰化酶抑制剂的作用机制,本实验以组蛋白去乙酰化酶抑制剂Trichostatin A处理小麦根尖,通过随机扩增多态性DNA标记技术(random amplified polymorphic DNA,RAPD)和双限制性内切酶酶切-随机扩增法(Coupled Restriction Enzyme Digestion-Random Amplification,CRED-RA)进行探究的结果发现,与对照组相比,处理组10个随机引物的RAPD扩增条带出现83条多样性差异,多样性比例达到87.4%,同时处理组DNA甲基化比例增加.本研究证实Trichostatin A能使小麦基因组DNA发生改变,并出现了表观修饰,从而从分子和表观水平探讨了该抑制剂的作用机理.In this paper,wheat（ Triticum aestivum L.） seeds was treated with Trichostatin A,a specific inhibitor of histone deacetylase. It was shown by Random Amplified Polymorphic DNA（ RAPD） with 10 random primers that here were 83 polymorphic bands in the treating groups compared to the controls. The polymorphic ratio reached87. 4%. Coupled Restriction Enzyme Digestion-Random Amplification（ CRED-RA） analysis indicated that DNA methylation increased in treatment groups. Our results demonstrate that Trichostatin A can not only change the wheat genomic DNA,but also increase the epigenetic modification and provide direct evidence on the mechanism of Trichostatin A function from molecular and epigenetic levels.

关 键 词：TRICHOSTATIN A 随机扩增多态性DNA标记技术 双限制性内切酶酶切-随机扩增法 DNA甲基化 小麦 

分 类 号：Q781[生物学—分子生物学]