不同剂量盐酸戊乙奎醚对创伤性休克兔肠组织iNOS活性和NO含量的影响  

Effect of different dose of penehyclidine hydrochloride on activity of iNOS and contents of NO in intestinal tissues of rabbits with traumatic shock

作　　者：梁鹏冲[1] 刘宗萍 王瑜清 张琼[1] 石亚军[1] 樊冬梅[1] 王大军[3] 王鹏[3] 王厚清[3] 赵宁军[3] 燕宪亮[3] 许铁[3] 

机构地区：[1]宝鸡市中心医院急诊科,陕西721008 [2]清姜社区卫生服务中心 [3]徐州医学院急救与救援医学系/徐州医学院附属医院急救中心

出　　处：《中国急救复苏与灾害医学杂志》2015年第11期1058-1062,共5页China Journal of Emergency Resuscitation and Disaster Medicine

基　　金：徐州市社会发展基金资助项目（XM08C084）

摘　　要：目的研究盐酸戊乙奎醚（PHC）对创伤性休克兔肠黏膜损伤的保护作用及对肠组织诱导型一氧化氮合酶（iNOS）活性和一氧化氮（N0）含量的影响。方法采用Lamson’s法建立创伤性休克模型，30只健康日本长耳大白兔，采用随机数字表分为对照组（Con组）、生理盐水复苏组（NS组）和PHC复苏组；后者又分为低剂量组（PHCL组）、中剂量组（PHCM组）和高剂量组（PHCH组），每组6只。分别在休克前（T1）、休克末（T2），复苏成功后即刻（B）、2h（T4）、4h（T5）和6h（T6）等6个时间点测量平均动脉压（MAP）和心率（HR），采血测定二胺氧化酶（DAO）活性；实验结束后处死动物，取小肠组织测定iNOS活性和NO含量，观察肠黏膜病理学变化。结果在T2时间点实验动物均达到MAP≤45mmHg的预期水平；T3～T6等时间点，3个PHC组和NS组兔的MAP均显著回升，但NS组兔的MAP回升幅度较小，与Con组和PHCM组相比，差异有统计学意义（P〈0．05）。在T3时间点3个PHC组和Ns组兔的HR显著减慢，与T1时间点相比差异有统计学意义（P〈0．05）；随后的3个时间点，3个PHC组兔的HR均明显回升，而NS组兔的HR进一步减慢，与Con组和PHCM组相比差异有统计学意义（P〈0．05）。在T2-T6等时间点，3个PHC组和NS组兔血浆DAO活性显著升高，与T1时间点、Con组相比差异有统计学意义（P〈0．05），DAO活性随时间呈双峰（T2和T5）改变；T3－T6等4个时间点，Ns组兔血浆DAO活性显著高于3个PHC组，PHCL组和PHCH组又显著高于PHCM组，差异均有统计学意义（P〈0．05）。3个PHC组和Ns组兔肠组织iNOS活性和NO含量显著升高，与Con组相比差异有统计学意义（P〈0．05）；NS组肠组织iNOS活性和NO含量高于3个PHC组、后两组又高于PHCM组，差异有统计学意义（P〈0．05）。病理组织学检查显示PHCM组肠黏膜损伤较Ns组显著减轻。结论PHC能稳Objective To investigate the protective effects of penehyclidine hydrochloride （PHC） on intestinal mucosa injury in rabbits with traumatic shock, the activity of inducible nitric oxide synthase （iNOS） and the concentratibn of nitric oxide （NO） in the intestinal tissue. Methods The model of traumatic shock was established by Lamson＇s method. Thirty healthy Japanese long ear albino rabbits were randomly divided into five groups （n=6 each）： control group （Con group）, traumatic shock resuscitation with normal saline group （NS group）, traumatic shock resuscitation treated with PHC in low dosage group （PHCL group）, middle dosage group （PHCM group） and high dosage group （PHCH group）. At pre-shock （T1）, the end of shock （T2）, 0 h （T3）, 2 h （T4）, 4 h （T5）, 6 h （T6） after resuscitation, mean arterial pressure （MAP） and heart rate （HR） were monitored. At the end of experiment, the animals were sacrificed and the samples were collected, iNOS activity and NO concentration in intestinal tissue were measured. The pathological changes of intestinal tissue were observed under microscope. Results At T1 time point, there were no significant differences in MAP among the five groups. At T2 time point, MAP of rabbits in 4 experimental groups were prospectively decreased to level （≤45 mmHg）; at T3-T6 time points, MAP of rabbits in three PHC groups and NS group were significantly increased, but MAP of rabbits in NS group rose to a lesser extent, the difference between MAP of rabbits in NS group and in Con group and PHCM group was statistically significant （P 〈0.05）. At T3 time point, HR of rabbits in three PHC groups and NS group was significantly decreased; then at three time points, HR of rabbits in three PHC groups rebounded significantly, but HR of rabbits in NS group slowed down further, the difference between HR of rabbits in NS group and in Con group and PHCM group was statistically significant （P 〈0.05）. Compared with Con group and T1 time

关 键 词：创伤性休克 盐酸戊乙奎醚 诱导型一氧化氮合酶 一氧化氮 

分 类 号：R605.971[医药卫生—急诊医学]