抗人CCR5多克隆抗体体外阻断HIV-1假病毒感染的研究  

Polyclonal antibodies to Human CCR5 Blocking the Infection of HIV-1 Pseudo-typed Virus in Vitro

作　　者：程林[1] 唐娴[1] 赵方[1] 何美云[1] 王辉[1] 

机构地区：[1]深圳市第三人民医院,广东深圳518112

出　　处：《中国艾滋病性病》2015年第11期916-918,921,共4页Chinese Journal of Aids & STD

基　　金：国家"十二五"传染病重大专项(2012ZX10001009-001-001)~~

摘　　要：目的制备鼠抗人CC型趋化因子受体5(CCR5)特异性Ⅰ型艾滋病病毒(HIV-1)中和抗体。方法用稳定表达人CCR5的CHO-R5细胞腹腔免疫BALB/c小鼠;流式细胞术的方法分析免疫血清对GHOST-R5细胞的结合;纯化免疫血清总IgG抗体,HIV-1假病毒中和试验分析血清总IgG的中和活性。结果流式细胞术的结果显示,CHO-R5细胞免疫组小鼠血清,对GHOST-R5结合的平均荧光强度显著高于对照组(P<0.001);HIV-1假病毒中和试验的结果发现,纯化的CHO-R5细胞组小鼠免疫血清总IgG,可抑制HIV-1假病毒在体外感染GHOST-R5细胞,抑制作用具有剂量依赖性,最高抑制率达到92%。结论成功诱导出具有中和HIV-1活性的鼠抗人CCR5的多克隆抗体。Objective To prepare CCR5-specific HIV-1neutralizing antibodies.Methods BALB/c mice were intraperitoneally immunized with human CCR5stable-expressing CHO-R5cells;flow cytometry analysis was conducted on the binding of sera from immunized mice with GHOST-R5cells;the neutralizing activity of purified total IgG from immunized mice to HIV-1infection was determined by HIV-1env-pseudotyped virus-neutralizing assay.Results The mean fluorescence intensity of the binding of GHOST-R5 cells with sera from mice of CHO-R5 cells immunized group was significantly higher than that of the control group（P 0.001）;and the neutralizing assay showed that the immunized group with the purified total IgG from mice of CHO-R5 cells effectively inhibited the infection of GHOST-R5 cells by HIV-1pseudo-typed virus in a dose-dependent manner,with the highest inhibition ratio of92%.Conclusions Mouse anti-human CCR5 polyclonal antibodies with HIV-1neutralizing activity are successfully induced.

关 键 词：CC型趋化因子受体5 中和抗体 艾滋病病毒1型 

分 类 号：R373.9[医药卫生—病原生物学]