双等色点光度法测定姜黄素-铁(Ⅱ)的稳定常数及试验方法改进  

Dual Isosbestic Points Spectrophotometric Determination of Stability Constant of Curcumin-Fe(Ⅱ)Complex and Modification of the Test Method

作　　者：高苏亚[1,2] 李华[2] 刘小丽[3] 张韫[1] 马荣妹 

机构地区：[1]西安医学院药学院,西安710021 [2]西北大学分析科学研究所,西安710069 [3]长安大学环境科学与工程学院,西安710054

出　　处：《理化检验（化学分册）》2015年第11期1511-1514,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金项目(21375105);陕西省教育厅科研计划项目(14JK1620);省级大学生创新训练项目(1647)

摘　　要：采用双等色点光度法测定姜黄素-铁(Ⅱ)络合物的稳定常数并对试验方法进行了改进。采用两种紫外-可见分光光度法设计试验方案,考察了姜黄素的光谱特性,获得姜黄素与铁(Ⅱ)的络合物吸收光谱中两个等色点,姜黄素-铁(Ⅱ)络合物的稳定常数为1.48×1011(pH 6.50),改进后试验方法的稳定常数为1.49×1011。Dual isosbestic points-spectrophotometry was applied to the determination of stability constant of curcumin-Fe（Ⅱ）complex and the testing method was improved.The spectral characteristics of curcumin were investigated by two experimental programs of UV-visible spectrophotometry.Two isosbestic points were found in the absorption spectra of curcumin-Fe（Ⅱ）complex.The stability constant of the curcumin-Fe（Ⅱ）complex was found as 1.48×1011（at pH 6.50）,and value of stability constant of complex of 1.49×1011 was obtained by the inproved method.

关 键 词：分光光度法 稳定常数 改进 姜黄素-铁(Ⅱ)络合物 

分 类 号：O657.32[理学—分析化学]