高效液相色谱法测定木通属植物苯乙醇苷B含量  

Phenylethanoid glycosides B content of Akebia plants determined by HPLC

作　　者：高伟[1,2] 幸伟年[1] 敖婉初[1] 徐林初[1] 龚春[1] 彭以元[2] 

机构地区：[1]江西省林业科学院,江西南昌330013 [2]江西师范大学,江西南昌330029

出　　处：《南方林业科学》2015年第5期48-51,共4页South China Forestry Science

基　　金：国家林业局2013年林业公益性行业科研专项(项目编号:201304802)"木通科特色蓇葖果良种选育及高效栽培技术研究"

摘　　要：以江西、湖南、四川、安徽4省的38份木通植物为试材,采用高效液相色谱法建立木通属植物苯乙醇苷B含量的测定方法,并测定木通藤茎中苯乙醇苷B的含量。结果表明：1）采用85%甲醇回流提取1 h,可以完整提取木通苯乙醇苷B;木通苯乙醇苷B在0.04~1μg呈良好的线性关系,R2=0.9991,平均回收率99.44%。2）木通苯乙醇苷B在三叶木通、五叶木通、白木通藤中均含有,含量0.01%~1.194%,其中在三叶木通中的成熟藤中含量最大。The method of determination phenylethanoid glycosides B content in Akebia plant had been researched. Use HPLC to determine the content of phenylethanoid glycosides B in 38 copies of A. stem which came from Jiangxi, Hu＇nan,Sichuan and Anhui Province. The results showed that：（1） Phenylethanoid glycosides B can be complete extracted by 85%methanol reflux extraction 1 h, and showed a good linear relationship in 0.04 ~ 1μ g when use HPLC,（R2=0.9991） and the average recovery was 99.44%.（2） Phenylethanoid glycosides B contained in the stem of A. trifoliata, A. quinata, A. trifoliata,fluctuated from 0.01% to 1.194%, and the content in the mature A. trifoliata was the largest.

关 键 词：木通苯乙醇苷B 三叶木通 江西 

分 类 号：S567[农业科学—中草药栽培] Q949.95[农业科学—作物学]