孕早、晚期接受七氟醚麻醉对大鼠子代大脑神经发育的影响  

Effect of sevoflurane inhalation anesthesia during early and late pregnancy on offspring brain neural development in rats

作　　者：陈弦[1] 陶凡[1] 黄丽霞[1] 范皓[1] 

机构地区：[1]杭州市红十字会医院麻醉科,310003

出　　处：《浙江医学》2015年第19期1570-1572,1581,共4页Zhejiang Medical Journal

基　　金：杭州市卫生科技计划项目(2012A044)

摘　　要：目的研究孕早、晚期Wistar大鼠母体接受七氟醚吸入麻醉对子代大脑神经发育的影响。方法将12只孕鼠按随机数字表法分为孕6d用药组(A组)、孕19d用药组(B组)和对照组(C组),每组各4只。A、B组分别于孕6d、孕19d时吸入2.4%七氟醚6h,C组饲养于空气条件下。应用Nissl染色法观察仔鼠在出生后第1、14、21天脑组织海马CA1区神经元尼氏体的表达情况。在仔鼠出生后第32天采用Morris水迷宫实验对各组仔鼠学习记忆功能进行测定。结果出生后第1天,B组仔鼠海马CA1区神经元损伤程度明显高于A组及C组仔鼠;出生后第14天,B组仔鼠神经损伤减轻,A组仔鼠神经损伤加重;出生后第21天,B组仔鼠神经损伤程度较轻,A组仔鼠神经损伤较重。出生后第32天,A组和B组仔鼠逃避潜伏期、穿越目标象限次数、穿越平台次数与对照组仔鼠相比均无统计学差异(均P>0.05)。结论在本实验条件下,孕早、晚期母体接受2.4%七氟醚麻醉对Wistar大鼠子代大脑神经发育的影响随时间变化而改变,并不造成子代远期学习记忆功能异常。Objective To investigate the effect of sevoflurane inhalation anesthesia during early and late pregnancy on offspring brain neural development in rats. Methods Twelve pregnant Wistar rats were randomly divided into group A（6-day gestation）, group B（19-day gestation） and group C（control group） with 4 animals in each group. The rats in groups A and B received 2.4% sevoflurane （0.5L/min） oxygen for 6 h at 6th and 19th day of pregnancy, respectively, while those in control group had no any treatment. The expression of Nissl body in the CA1 region of hippocampus of the offspring at postnatal d1, d14 and d2t were observed with Nissl staining technique. The learning and memory function of the offspring was tested by Morris water maze test at postnatal d32. Results At postnatal dl, the injury in the CA1 region of hippocampus of the offspring in group B was more serious than that in group A and C. At postnatal d14, the neural injury of the offspring in group B was alleviated, but that in group A was worse. At postnatal d 21, the neural injury of the offspring in group B was mild, but that in group A was serious. There were no significant differences in escape latency, explore number in the target quadrant and platform of among the off- spring of groups A, B and C （P 〉0.05）. Conclusion The effect of sevoflurane inhalation during early and late pregnancy on off- spring brain neural development is time-dependent, but has no significant effect on the learning and memory function.

关 键 词：七氟醚 子代 学习记忆 尼氏体 孕早期 孕晚期 大鼠 

分 类 号：R614[医药卫生—麻醉学]