皖北地区仔猪源大肠杆菌毒力因子及HPI毒力岛的检测  

Detection of virulence factors and HPI pathogenicity island of Escherichia coli associated with diarrhea from piglets in Northern Anhui

作　　者：吕锦芳[1] 闻爱友[1] 宁康健[1] 姜锦鹏[1] 王璇[1] 路振香[1] 刘畅[1] 应如海[1] 孙浩楠 王稳[1] 

机构地区：[1]安徽科技学院动物科学学院,安徽凤阳233100

出　　处：《中国兽医学报》2015年第11期1782-1785,1791,共5页Chinese Journal of Veterinary Science

基　　金：安徽蚌埠市农业科技计划基金资助项目(2013)

摘　　要：我国皖北地区哺乳仔猪腹泻频发,临床直肠棉拭子培养物有34例符合大肠杆菌特征,并进行产肠毒素大肠杆菌(ETEC)毒力因子(STa、STb、LT、SLT-2e)和HPI毒力岛的PCR快速检测。结果,仅有10例表达STa和STb(29.41%),其中3例表达STa基因(8.82%),2例表达STb基因(5.88%),2例表达STa和STb基因,1例表达STa和irp2基因(2.94%),2例表达STa和STb及irp2基因,揭示HPI+大肠杆菌与ETEC混合感染共有3例(8.82%);未检测到LT和STL-2e毒力因子。有10例表达irp2基因(29.41%),其中7例独立表达irp2基因(20.59%)。STa、STb和Irp2的PCR产物测序结果分别与GenBank检索的目标序列比对,其同源性均达到100%。结果初步揭示了皖北地区致哺乳仔猪腹泻ETEC毒力因子及HPI毒力岛的分布情况。The piglet diarrhea often occurs in Northern Anhui.In 34 cases of clinical rectal swab culture with Escherichia coli characteristics,and virulence factors（STa,STb,LT,SLT-2e）of Eeterotoxigenic Escherichia coli（ETEC）and HPI pathogenicity island were tested by rapid PCR.The results showed that among them,only 10 cases sample could express STa and STb（29.41%）,the expression of STa gene were 3cases（8.82%）2cases（5.88%）with STb,2cases for STa and STb gene,1case for,STa and irp2gene（2.94%）,2cases for STa,STb and irp2.The Escherichia coli of HPI-positive and ETEC mixed infection were 3cases（8.82%）.LT and STL-2evirulence factors was not detected.A total of 10cases（29.41%）expressed irp2 gene,the expression of irp2 gene were 7cases（20.59%）among them.PCR product sequencing results of STa,STb and Irp2 were comparied with the target sequences of GenBank respectively,the homology were all up to 100%.These results indicate that the distribution of virulence factors of ETEC and HPI pathogenicity island could induce piglet diarrhea in localarea of Northern Anhui.

关 键 词：仔猪腹泻 大肠杆菌 毒力因子 HPI毒力岛 

分 类 号：S852.61[农业科学—基础兽医学]