鸡NLRC5基因启动子多态性分析  

Polymorphism analysis of NLRC5 gene promoter of chickens

作　　者：郭晓敏[1] 刘向萍[2] 常国斌[1] 徐璐[1] 马腾[1] 王洪志[1] 李志腾[1] 陈静[1] 万方[1] 徐琪[1] 陈国宏[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]中国农业科学院家禽研究所,江苏扬州225001

出　　处：《中国兽医学报》2015年第11期1845-1849,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31301966;31172199);江苏省农业科技支撑计划资助项目(BE2013392)

摘　　要：为了研究NLRC5基因启动子区的SNP及其对启动子功能元件的影响,采用目标捕获测序和PCR产物直接测序的方法对27个鸡品种和2种细胞的NLRC5基因启动子区进行SNP检测,总共检测到37个SNP位点,其中斗鸡未发现SNP存在,SNP9存在于除斗鸡以外的其他鸡种和细胞。生物信息学软件预测得到NLRC5基因启动子区转录因子结合位点和CpG岛,SNP位点对转录因子结合位点和CpG岛大小均有影响,表明NLRC5启动子区SNP可能通过不同方式影响基因表达调控。To study the SNP in NLRC5 gene promoter and its effect on the promoter functional elements,target sequence capture assay and PCR direct sequencing were used to detect SNP in NLRC5 gene promoter of 27 chicken breeds and two kinds of cells.There were 37 SNP loci totally in all breeds and cells,but no SNP exist in gamecock breed.SNP9 present in all chicken breeds and cells except gamecock.Moreover,we used bioinformatics software to predict transcription factor binding sites and CpG islands in NLRC5 gene promoter,and found that SNP influenced the transcription factor binding sites and CpG island size,suggesting that SNP in NLRC5 promoter may had effects on the regulation of gene expression through different ways.

关 键 词：鸡 NLRC5基因 启动子 SNP 

分 类 号：S831[农业科学—畜牧学]