荷斯坦奶牛妊娠早期血浆差异蛋白质组学研究  

Differential proteomic analysis of blood plasma in early pregnancy of Holstein cows

作　　者：刘姗姗[1] 陶金忠[2] 武小虎[3] 赵国顺 杨永新[5] 赵兴绪[3] 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]宁夏大学农学院,宁夏银川750021 [3]甘肃农业大学动物医学学院,甘肃兰州730070 [4]天水市秦安县畜牧兽医局,甘肃天水741600 [5]安徽农业科学院畜牧兽医研究所,安徽合肥230031

出　　处：《中国兽医学报》2015年第11期1850-1855,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31160523)

摘　　要：采用二维凝胶电泳(2-DE)结合MADIL-TOF-TOF串联质谱法对荷斯坦奶牛妊娠早期血浆进行蛋白质组学分析鉴定,发现了6种差异蛋白在妊娠后表达量上调,分别是凝集素1前体、转甲状腺素蛋白前体、3,5-二碘水杨酸络合的牛血清白蛋白的晶体结构的A链、白蛋白、未命名蛋白产物及结合珠蛋白-25同源物1前体。对这6种蛋白在不同时期含量的变化趋势及可能引起每种蛋白含量变化的原因和在妊娠过程中的功能进行了分析。Proteome analysis in plasma from Holstein cows in early pregnancy were performed using two-dimensional gel electrophoresis（2-DE）method combined with MADIL-TOF-TOF tandem mass spectrometry.Six proteins were up-regulated after breeding,including TPA：conglutinin 1precursor,transthyretin precursor,Chain A,Crystal Structure Of bovine serum albumin in complex with 3,5-diiodosalicylic acid,ALB protein,unnamed protein product and protein HP-25 homolog 1precursor.The content variation tendency of the six proteins in different periods were analyzed.The reasons about causing the content changes of each protein and functions of six proteins during pregnancy were discussed.

关 键 词：荷斯坦奶牛 妊娠 血浆 蛋白组 

分 类 号：S823.91[农业科学—畜牧学]