16S rDNA-RFLP分析不同产地人参内生菌的多态性  

Analysis the Differences of Endophyte Bacterias in Panxiginsengs by 16S rDNA – RFLP Method

作　　者：万红娇[1] 谢燕飞[1] 谢斌[1] 朱金华[1] 马广强[1] 

机构地区：[1]江西中医药大学基础医学院,南昌330004

出　　处：《江西中医药大学学报》2015年第5期51-54,共4页Journal of Jiangxi University of Chinese Medicine

基　　金：江西省卫生厅中医药科研计划项目(2012A034)

摘　　要：目的:采用16S r DNA—RFLP方法分析比较不同产地、品种人参内生菌的差异,为探讨人参内生菌对其药材的影响奠定基础。方法:收集不同产地、不同品种人参,用细菌基因提取试剂盒提取人参内生菌基因组,扩增内生菌16S r DNA片段后,用RFLP(限制性酶切片段分析)方法分析不同产地、不同品种人参内生菌的多态性。结果:不同产地、不同品种人参均含有内生细菌,且内生细菌菌种组成有明显差异。结论:不同产地的人参有不同的人参内生菌,通过鉴定内生菌可鉴定人参的产地。Objective： In this study,method of 16 S Rdna- RFLP were used to detect the differences of the endophyte in Panxginseng.Methods： PCR were used to amplify the 16 S r DNA of endophyte bacterias in Panxginseng,then the products of PCR were digested by single enzyme. Results： there Were obvious differences in the endophyte bacterias in different kinds of Panxginsengs. Conclusion： It is possible to identify the producing region of Panxginsengs by detecting the special bacterias in its endophte.

关 键 词：人参 16S RDNA RFLP 内生菌 

分 类 号：Q93[生物学—微生物学]