α7nAChR基因敲除小鼠的饲养繁育及基因型鉴定  

Reproduction and Genotype Identification of α7nAChR Knockout mice

作　　者：李富强[1] 吴小翠[1] 陈小梅[1] 唐翠兰[2] 

机构地区：[1]浙江中医药大学第二临床医学院,杭州310053 [2]浙江省新华医院感染科,杭州310006

出　　处：《江西中医药大学学报》2015年第5期75-77,86,共4页Journal of Jiangxi University of Chinese Medicine

基　　金：国家自然科学基金项目(81100279);浙江省大学生科技创新活动计划暨新苗人才计划项目(2014R410058)

摘　　要：目的:繁殖和鉴定烟碱型乙酰胆碱受体α7基因敲除小鼠。方法:将引进的α7n AChR基因敲除杂合子小鼠进行饲养并繁殖,提取子代小鼠尾部DNA,用PCR方法检测子代小鼠基因型;采用Western blot检测α7n AChR蛋白质的表达,进一步证实PCR鉴定方法的可靠性。结果:获得子代(α7n AChR+/+),子代小鼠纯合率为30%。结论:α7n AChR基因敲除小鼠的繁育和鉴定均获得成功,为进一步研究α7n AChR缺失对非酒精性脂肪性肝炎的影响提供了理想的动物模型。Objective： Reproduce and identify α7n AChR knockout mice. Methods： Heterozygote mice were bred and reproduced. Genome DNA was extracted from the murine tail and identified by PCR; Additionally,the expression of α7n AChR protein was detected by Western blot to further confirmed the reliability of PCR methods for genotype identification of α7n AChR mutant mice. Results：α7n AChR genotypes of the offspring mice included heterozygous（ α7n AChR ＋ /-）,homozygous（ α7n AChR- /-）,and wild types（ α7n AChR ＋ / ＋）. The homozygous rate of offspring was 30%. Conclusion： α7n AChR knockout mice are successful reproduced,which provides an ideal animal model for study of the NASH.

关 键 词：烟碱型乙酰胆碱受体α7 基因敲除小鼠 非酒精性脂肪性肝炎 

分 类 号：R285.5[医药卫生—中药学]