应用实时荧光定量PCR技术检测土壤中根肿病菌休眠孢子  

Application of real-time fluorescent quantitative PCR for detection of resting spores of Plasmodiophora brassicae in field soil

作　　者：宋琼[1,2] 任佐华[1,2] 杨华[1,2] 刘二明[1,2] 

机构地区：[1]湖南农业大学植物保护学院,湖南长沙410128 [2]植物病虫害生物学与防控湖南省重点实验室,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2015年第6期631-635,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：公益性行业(农业)科研专项(201003029)

摘　　要：为准确、快速、便捷检测出土壤中根肿病菌休眠孢子量,应用所建立的根肿病菌实时荧光定量聚合酶链式反应检测技术,测定了湖南湘潭县响水乡、长沙县路口镇、桃江县桃花江镇十字花科作物根肿病发生地的42份土样的休眠孢子数量。检测结果表明,40份土样的根肿病菌休眠孢子量为（2.76×104~4.37×106）个/g;休眠孢子量≥104个/g的土壤均有根肿病发生,而休眠孢子量为8.42×102、9.78×102个/g的2份土样未发生根肿病,说明当土壤中根肿病菌休眠孢子量≥104个/g时,根肿病害易发生。To detect the content of Plasmodiophora brassicae （P. brassicae） resting spores in soil samples accurately, quickly and conveniently, a real-time fluorescent quantitative PCR was applied to detect the content of resting spores of P. brassicae in 42 soil samples which collected from Xiangshui township in Xiangtan county, Lukou town of Changsha county and Taohuajiang town of Taojiang county. The results showed that the number of resting spores per gram soil in 40 of 42 soil samples were between 2.76× 104 to 4.37× 106, in which the samples with resting spores≥ 104/g soil all had clubroot occurrence while the 2 samples with 8.42×102 and 9.78 × 102 resting spores per gram soil didn＇t have. Therefore, occurrence risk of the clubroot was large when the content ofP. brassicae resting spores ≥ 104/g soil.

关 键 词：十字花科作物 根肿病菌 休眠孢子量 荧光定量PCR 检测 

分 类 号：S436.34[农业科学—农业昆虫与害虫防治] Q789[农业科学—植物保护]