猕猴脾脏中IL–2蛋白表达的性别差异  

Difference of IL–2 expression in spleen of macaque between the male and female

作　　者：马文静[1] 罗启慧[1,2] 陈姗姗[1] 陈正礼[1,2] 

机构地区：[1]四川农业大学动物医学院动物疾病与人类健康中心四川省重点实验室,四川雅安625014 [2]四川农业大学预防兽医研究所,四川成都611130

出　　处：《湖南农业大学学报（自然科学版）》2015年第6期651-654,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家科技支撑计划项目(2014BAI03B01);四川省青年科技创新研究团队项目(2013TD0015)

摘　　要：观察白细胞介素2(IL–2)在猕猴脾脏中的表达分布及性别差异。采用免疫组织化学SP法,对雌、雄猕猴脾脏内IL–2的分布与表达进行观察。雄性猕猴脾脏红髓区阳性细胞强染且多为胞浆着色,中等密度分布;白髓区有低密度分布的中等阳性细胞,且多数为胞浆着色;在边缘区有中等密度分布的中等阳性细胞。雌性猕猴脾脏中,红髓区脾索有高密度强阳性细胞,有部分胞核和部分胞浆着色;白髓区有低密度分布的胞核着色的中等阳性细胞;边缘区有中等密度分布的中等阳性细胞。雌性猕猴脾脏中IL–2阳性细胞数量及蛋白表达强度均显著高于雄性猕猴(P<0.01或0.05)。IL–2阳性产物主要表达于红髓中,表明IL–2对猕猴脾脏体液免疫功能的发挥起到了较强的作用,且性别差异明显。Interleukin-2 was observed via SABC, an immunohistochemical method, to investigate its expression and distribution in macaque＇s spleen and its difference with sex. Positive cells within red pulp area in spleen of male macaque were strongly stained with cytoplasm with a medium density distribution. The majority of middle positive cells with a low-density distribution in white pulp area were stained with cytoplasm. The middle-density middle positive cells were distributed in the marginal region. The high-density of strong positive cells were found in the red pulp area of splenic cord in the spleen of female macaque, some nuclei were stained, while, others were stained with cytoplasm. Middle positive cells with nuclei stained had low-density distribution in the white pulp area. The middle-density middle positive cells were distributed in the marginal region. In short, the interleukin-2 in spleen of female macaque was apparently higher than that in males both on the number of positive cells and protein expression intensity （P〈0.01 or 0.05）. Higher expression of the interleukin-2 in red pulp area suggested that it played an important role in enhancing the humoral immune function, and this depended greatly on sex.

关 键 词：猕猴 脾脏 白细胞介素 

分 类 号：S865.16[农业科学—野生动物驯养]