戊唑醇和吡唑醚菌酯在玉米植株和土壤中残留及其消解动态的检测  

Detection the residues and their dynamic of tebuconazole and pyraclostrobin in maize straw and soil

作　　者：傅强[1] 聂思桥[1] 任竞[1] 梁骥[1] 付启明[1] 刘金胜[1] 刘义珂[1] 

机构地区：[1]湖南化工研究院有限公司,湖南长沙410014

出　　处：《湖南农业大学学报（自然科学版）》2015年第6期661-665,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：农业部农药检定所项目(HN13–07)

摘　　要：采用高效液相色谱–串联质谱（LC–MS/MS）方法,分析戊唑醇和吡唑醚菌酯在玉米植株和土壤中的残留及消解动态。土壤样品采用乙腈提取,植株样品采用乙腈和丙酮提取,过膜后采用LC–MS/MS分析。结果表明：戊唑醇和吡唑醚菌酯在土壤和玉米植株中的定量限分别为0.01、0.02 mg/kg,检出限分别为0.001、0.005 mg/kg。当添加水平为0.01~2.00 mg/kg时,戊唑醇和吡唑醚菌酯在土壤和玉米植株中的平均回收率为83.9%~113.3%,相对标准偏差为1.0%~8.0%。消解动态试验结果表明：30%戊唑醇·吡唑醚菌酯悬浮剂按1 050 g/hm2（有效成分315 g/hm2）于玉米苗期施药1次,戊唑醇和吡唑醚菌酯在土壤及玉米植株中的消解动态规律均符合一级动力学方程曲线,戊唑醇在玉米植株和土壤中的消解半衰期分别为5.22、14.10 d,吡唑醚菌酯在玉米植株和土壤中的消解半衰期分别为4.78、13.40 d,二者均属易消解型农药。The residual dynamic of tebuconazole and pyraclostrobin in maize straw and soil were measured by liquid chromatography and tandem mass spectrometry （LC-MS/MS）. Residues of two chemicals in soil samples extracted with acetonitrile, and maize straw samples extracted with acetonitrile and acetone, were analyzed by LC-MS/MS. The limits of quantification （LOQs） of tebuconazole and pyraclostrobin in maize straw and soil were 0.02 mg/kg and 0.01 mg/kg, respectively. The limits of detection （LODs） in maize straw and soil were 0.005 mg/kg and 0.001 mg/kg, respectively. When the fortified level of tebuconazole and pyraclostrobin ranged from 0.01 to 2.00 mg/kg,the average recoveries of two compounds varied from 83.9% to 113.3% with the relative standard deviation varied from 1.0% to 8.0%. When the maize straw and soil were sprayed with 30% tebuconazole and pyraclostrobin suspension concentrate（SC） at a dosage of 1 050 g/hm2in maize seedling stage,the half-life time of tebuconazole in maize straw and soil were 5.22 d and 14.10 d respectively, and that of pyraclostrobin were 4.78 d and 13.4 d respectively. In conclusion,tebuconazole and pyraclostrobin belong to the easily degradable pesticide types.

关 键 词：戊唑醇 吡唑醚菌酯 液相色谱–串联质谱 玉米植株 土壤 消解动态 

分 类 号：TQ450.2[化学工程—农药化工]