糖尿病大鼠模型的建立及相关蛋白组学的实验研究  

Establishment of the diabetic rat models and analysis of serum proteomics

作　　者：陈红谨[1] 刘小溪[2] 连捷 杨宇峰[4] 石岩[4] 

机构地区：[1]山西中医学院,山西太原030024 [2]辽宁中医药大学附属医院,辽宁沈阳110032 [3]大连市骨科医院,辽宁大连116011 [4]辽宁中医药大学,辽宁沈阳110847

出　　处：《山西中医学院学报》2015年第4期25-28,44,共5页Journal of Shanxi College of Traditional Chinese Medicine

摘　　要：目的:研究糖尿病大鼠模型的建立及其蛋白组学的变化,从蛋白质的分子水平上探索糖尿病发生的病理生理机制。方法:用高脂高糖饮食+链脲佐菌素的方法建立糖尿病大鼠模型,采用同位素标记技术,利用高效液相色谱仪及四级杆-静电场轨道阱质谱仪,对糖尿病大鼠进行串级质谱数据分析,对检测出的有统计学意义的表达差异蛋白进行研究。结果:第2周开始,模型组与正常组的血糖差异有统计学意义(P<0.05)。第10周,模型组血糖基本达到了糖尿病模型的标准,空腹血糖>16.7 mmol/L;蛋白组学检测共获得有显著差异的蛋白50个,其中上调大于1.5倍的蛋白17个,主要有MHCⅡ反式激活因子、主要尿蛋白、LIM结构域蛋白等;下调小于0.75倍的50个,主要有肉碱乙酰转移酶、多聚免疫球蛋白受体前体等。结论:建立了相对符合糖尿病发生发展规律的糖尿病大鼠模型,血清蛋白组学分析糖尿病发生的机制中存在免疫系统、糖代谢、脂代谢紊乱,从蛋白分子的水平研究糖尿病发生的病理生理基础,具有重要学术和临床意义。Objective：To study the establishment of the diabetic rat models and the change of serum proteomics so as to exploring the pathophysiological mechanism of diabetes from the level of protein molecules. Methods：The diabetic rat models were established by streptozotocin（STZ）and high fat-glucose diet. The serum proteomics was analyzed with isotope internal standard dilution technique,high performance liquid chromatography/tandem mass spectrometry （LC-MS/MS）. Results：From the second week,blood glucose in model group was significantly higher than that in normal group（P〈0.05）. For tenth week,the blood glucose of model group had basically reached the standard of diabetes model （FPG〉16.7 mmol/L）. There was significant difference in expression of 50 kinds of proteins between normal group and model group （17 kinds of proteins were up-regulated more than 1.5 times,including MHCⅡ transactivator,major urinary protein,LIM domain protein,etc;50 kinds of proteins down-regulated less than 0.75 times,including carnitine acetyltransferase,polymeric immunoglobulin receptor precursor,etc）. Conclusion：The diabetic rat models were established successfully. Pathological mechanism of diabetes may be related to disorders of immune and glucolipid metabolism.

关 键 词：糖尿病 链脲佐菌素 蛋白组学 动物模型 

分 类 号：R517[医药卫生—内科学]