缺氧诱导因子-1α在鼠肺缺血再灌注损伤中的表达与意义  

Expression and Significance of Hypoxia-inducible Factor-1α in Rat Lung Ischemia-reperfusion Injury

作　　者：程阳[1] 赵丽妮[2] 商丽宏[3] 

机构地区：[1]沈阳医学院病理生理学教研室,辽宁沈阳110034 [2]沈阳医学院药理学教研室,辽宁沈阳110034 [3]沈阳医学院形态与机能中心实验室,辽宁沈阳110034

出　　处：《中外医疗》2015年第19期8-9,12,共3页China & Foreign Medical Treatment

摘　　要：目的探讨缺氧诱导因子-1α(HIF-α)在鼠肺缺血再灌注损伤中的表达及其意义。方法采用沈阳医学院实验动物中心24只健康雄性大鼠,将大鼠分为A、B、C3组,A组在建立缺血再灌注损伤模型前24 h,给予腹腔内注射生理盐水;B组注射DMOG;C组仅左侧开胸,不行缺血再灌注处理,建立缺血再灌注损伤模型。结果 A组可见明显肺组织损伤;A、B组各时间HIF-1α蛋白表达增强,平均灰度值降低;A、B组1 h与6 h的IL-8与丙二醛含量均升高,超氧化物歧化酶活力下降;A组与B组再灌注后6 h,IL-8含量升高,丙二醛含量升高,超氧化物歧化酶活力下降,差异有统计学意义(P<0.05)。结论可通过调节HIF-1α活性达到保护缺血再灌注性肺脏,为治疗肺缺血再灌注损伤提供新的思路。Objective To discuss the expression and significance of hypoxia-inducible factor-1α （HIF-α） in rat lung ischemia-reperfusion injury. Methods 24 healthy male rats from Shenyang Medical College Experimental Animal Center were divided into three groups, group A, group B and group C. 24h before the establishment of ischemia-reperfusion injury model, group A were giv-en intraperitoneal injection of normal saline, group B were given the injection of DMOG, group C were only given left thoracotomy without ischemia-reperfusion treatment. Results Group A showed significant lung injury; the expression of HIF-1α protein in-creased and the average gray value decreased in group A and group B at each time point; the IL-8 and MDA content increased and SOD activity decreased in group A and group B at 1h and 6h after the reperfusion; the IL-8 and MDA content increased and SOD activity decreased in group A and group B 6h after the reperfusion（P〈0.05）. Conclusion Regulation of the activity of HIF-1αcan protect the ischemia-reperfusion lung, which provides a new idea for the treatment of lung ischemia-reperfusion injury.

关 键 词：缺氧诱导因子-1Α 肺缺血再灌注损伤 超氧化歧化酶 

分 类 号：R692[医药卫生—泌尿科学]