Enzyme-free amplified detection of micro RNA using target-catalyzed hairpin assembly and magnesium ion-dependent deoxyribozyme  

作　　者：Jinfeng Chen Bo Liu Xiaorong Song Ping Tong Huanghao Yang Lan Zhang 

机构地区：[1]Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education [2]Key Laboratory of Analysis and Detection for Food Safety of Fujian Province [3]College of Chemistry, Fuzhou University [4]Longyan Entry-Exit Inspection and Quarantine Bureau of P.R.C [5]Testing Center, the Sport Science Research Center, Fuzhou University [6]Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules (Yanbian University), Ministry of Education

出　　处：《Science China(Information Sciences)》2015年第12期1906-1911,共6页中国科学（信息科学）（英文版）

基　　金：supported by the National Natural Science Foundation of China(21275029);the Science and Technology Planning Project from AQSIQ(2014IK260);the National Basic Research Program of China(2010CB732403);the"12th Five-Year National Science and Technology Support Program"(2012BAD29B06);the Program for Changjiang Scholars and Innovative Research Team in University(IRT1116);the Teachers Educational Research Programme of Fujian Province(JA 14055)

摘　　要：As micro RNAs（mi RNAs） are aberrantly expressed in a variety of cancers, detecting them precisely is of great importance. Here we constructed a sensitive and selective enzyme-free sensing platform for mi RNA detection based on target-catalyzed hairpin assembly and magnesium ion-dependent deoxyribozyme（Mg^2＋-dependent DNAzyme）. This sensing method introduces two amplification circuits simultaneously and shows a low detection limit of 1 pmol/L. This enzyme-free method is especially preferred because of its facility and economy. Furthermore, this amplified sensor shows high selectivity for discriminating perfectly complementary target and other mismatched RNAs. Therefore, the established strategy could be used as a simple, sensitive and selective method for target mi RNA detection.As micro RNAs（mi RNAs） are aberrantly expressed in a variety of cancers, detecting them precisely is of great importance. Here we constructed a sensitive and selective enzyme-free sensing platform for mi RNA detection based on target-catalyzed hairpin assembly and magnesium ion-dependent deoxyribozyme（Mg^2＋-dependent DNAzyme）. This sensing method introduces two amplification circuits simultaneously and shows a low detection limit of 1 pmol/L. This enzyme-free method is especially preferred because of its facility and economy. Furthermore, this amplified sensor shows high selectivity for discriminating perfectly complementary target and other mismatched RNAs. Therefore, the established strategy could be used as a simple, sensitive and selective method for target mi RNA detection.

关 键 词：micro RNA enzyme-free amplified detection target-catalyzed hairpin assembly magnesium ion-dependent deoxyribozyme 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置] Q523[自动化与计算机技术—控制科学与工程]