TEKT3在特发性弱精子症患者精子中的表达  

Expression of TEKT3 in spermatozoa of idiopathic asthenozoospermia patients

作　　者：李玉山[1] 陈河涛 杨夕阳 王全先[1] 刘军杰[1] 王琳凯[1] 苏彦华[1] 孙琳[1] 

机构地区：[1]郑州大学第三附属医院人类精子库,郑州450052

出　　处：《郑州大学学报（医学版）》2015年第6期843-846,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：郑州市科技攻关计划项目112PPTSF318-7

摘　　要：目的:探讨特发性弱精子症患者精子中TEKT3表达的特点。方法:采用非连续密度梯度离心法分离、纯化正常对照组和弱精子症组精子标本各35份,分别采用RT-PCR和Western blot检测精子中TEKT3 mRNA和蛋白的表达。结果:与正常对照组比较,弱精子症组TEKT3 mRNA和蛋白表达均显著降低(t=9.610、10.090,P<0.001),相关性分析显示弱精子症组精子TEKT3 mRNA和蛋白表达水平与前向运动精子率呈正相关(r=0.684,P=0.001;r=0.483,P=0.013)。结论:TEKT3表达降低可能在特发性弱精子症发病中具有重要作用。Aim：To investigate the function of TEKT 3 protein in the pathogenesis of patients with idiopathic astheno-zoospermia .Methods：Semen samples were collected from 35 normal controls and 35 idiopathic asthenozoospermia patients and purified using Percoll discontinuous density gradients , then the expression of TEKT 3 mRNA and TEKT3 protein were detected by RT-PCR and Western blot .Results： Compared with normal controls , the expressions of TEKT3 mRNA and protein in spermatozoa of idiopathic asthenozoospermia group were obviously decreased （t=9.610,10.090,P〈0.001）. Correlation analysis revealed that the expressions of TEKT 3 mRNA and protein were positively correlated with spermatozoa progressive motility（r=0.684,P=0.001;r=0.483,P=0.013）.Conclusion： The reduced expression of TEKT3 may play an important role in the pathogenesis of idiopathic asthenozoospermia .

关 键 词：TEKT3 特发性弱精子症 精子前向运动能力 

分 类 号：R698.2[医药卫生—泌尿科学]