慢性间歇低氧并高血压SD大鼠模型的建立  

A hypertension rat model induced by chronic intermittent hypoxia

作　　者：蒋丽君[1] 刘建红[1] 宋玮 潘凤锦 全志豪 莫晓云[1] 雷志坚[1] 梁碧芳[1] 唐华林[1] 谢宇萍[1] 龙胜泽[1] 吴思旸 

机构地区：[1]广西壮族自治区人民医院广西睡眠呼吸疾病中心,南宁530021

出　　处：《中国临床新医学》2015年第11期1013-1015,共3页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：国家自然科学基金资助项目(编号:81160014);广西自然科学基金资助项目(编号:2014GXNSFAA118261)

摘　　要：目的建立慢性间歇低氧并高血压SD大鼠模型,为研究阻塞性睡眠呼吸暂停低通气综合征(OSAHS)并高血压的发病机制提供简单可复制的动物模型。方法氧舱系统分为控制系统、气源系统、暴露舱三个部分。选8~10周龄SD雄性大鼠24只(200~250 g)随机分成间歇低氧干预组和对照组。间歇低氧干预组12只暴露于间歇低氧舱内,白天给予间歇低氧干预(6%~8%的O_2 40 s/21%的O_2 80 s,如此往复循环8 h);对照组12只暴露于常氧舱。在安静状态下检测大鼠尾动脉收缩压,每只连续测量3次,取平均值。结果实验第35天,间歇低氧干预组尾动脉收缩压[(136.21±32.46)mmHg]高于对照组[(121.37±35.37)mmHg](P<0.05)。结论控制精确气源通断时间,可有效地模拟间歇低氧过程,重复性佳,是研究间歇低氧所致高血压机制的理想实验模型。Objective To establish a hypertension rat model induced by chronic intermittent hypoxia, and to provide a simple and duplicate animal model for the study of the pathogenesis of hypertension in OSAHS. Methods The intermittent hypoxia system was used to conduct the experiment, including the control subsystem, the gas supply subsystem and the exposed cabin. 24 male Sprague-Dawley rats, aged 8 to 10 weeks, and weighted from 200 to 250 g were randomly divided into two groups ： the intermittent hypoxia intervention group and the control group. The inter- mittent hypoxia intervention group（n = 12） was exposed to 6% to 8% oxygen for 40 seconds and then to 21% oxy- gen for 80 seconds in the exposed cabin for 8 hours per day with many intermittent hypoxia cycles. The control group （n = 12） was exposed to certain concentration oxygen chamber. The tail artery systolic pressure of the rats was taken at rest states for three consec^ltive times and the average was calculated. Results The intermittent hypoxia interven- tion group had a significantly higher systolic blood pressure than the control group at the 35th day of the experiment [（136.21 ± 32. 46 ） mmHg vs （121.37 ±35.37）mmHg, P〈0.05]. Conclusion Precisely aerating the oxygen on and off in an certain range of concentration can effectively make a simulated intermittent hypoxia process, which is an ideal animal model for studying the mechanisms of intermittent hypoxia-induced hypertension.

关 键 词：间歇低氧 高血压 SD大鼠 

分 类 号：R544.1[医药卫生—心血管疾病]