Study on Rooting of Strawberry Bechika  

“贝吉佳”草莓脱毒苗生根及驯化研究（英文）

作　　者：胡婷婷[1] 利爽[1] 吕天舒[1] 高方可 吴荣哲[1] 

机构地区：[1]延边大学农学院园艺园林系,吉林延吉133002

出　　处：《Agricultural Science & Technology》2015年第11期2412-2415,2440,共5页农业科学与技术（英文版）

摘　　要：[Objective] The aim was to study rooting environment of strawberry ＂Bechi- ka＂ seedlings, and the somaclone domesticated transplanting was carded on. [Method] With the stem tip of stolon as material, the influence factom of rooting and the effects of different transplanting substrata on survival rate was investigated. [Result] The results showed that： 1/2MS was the best concentration for rooting and the rooting rate could reach 100%. IBA was better than IAA for rooting on growth regu- lator levdls, and it had the best effect on rooting with 0.3 mg/L, which produced the thickest and most-fibrous roots. Activated carbon had positive influences on the growth of roots, and the suitable concentration of activated carbon was 0.5 mg/l_ for rooting. The study found that survival rate of tissue culture seedlings was obviously higher in the combination of perlite and vermiculite than that in the combination of humus and sand, and the survival rate could reach 98.1% （perlite：vermiculite=l：l）. Seedlings in various transplanting substrata could normally bloom, fruit and form stolons. [Conclusion] The study provided technical support for achieving large-scale production of virus-free strawberry and quickly cultivating good seedlings.[目的]研究"贝吉佳"草莓组培生根环境,并对组培苗进行驯化移栽。[方法]以"贝吉佳"草莓匍匐茎茎尖为外植体,研究其生根的影响因素及移栽基质对成活率的影响。[结果]最佳的生根培养基浓度为1/2MS,生根率可达100%;IBA的生根效果优于IAA,当IBA浓度为0.3 mg/L时生根效果最好,根粗壮,须根最多:活性炭浓度为0.5 g/L,最适合"贝吉佳"草莓根的生长。组培苗的驯化中,珍珠岩和蛭石组合基质的成活率明显高于腐殖土和沙子的基质组合,成活率最高可达98.1%(珍珠岩:蛭石=1:1),在各种基质上的苗均可以正常开花结果、正常抽生匍匐茎。[结论]为实现"贝吉佳"草莓脱毒苗的规模化生产和快速培育优良种苗提供技术支持。

关 键 词：Strawberry ＂Bechika＂ MS concentrotion AUXINS Activated carbon Transplanting substratum 

分 类 号：S668.4[农业科学—果树学]