富氢水预处理对预防大鼠肝脏缺血再灌注损伤的病理学研究  

The pathology research of hydrogen-rich suline preconditioning on hepatic ischemia-reperfiision injury in rats

作　　者：孟令国[1] 王新立[1] 孙丰伟[1] 

机构地区：[1]泰山医学院附属医院,山东泰安271000

出　　处：《国际检验医学杂志》2015年第A02期13-16,共4页International Journal of Laboratory Medicine

摘　　要：目的探讨富氢水预处理后大鼠肝脏缺血再灌注损伤的病理学改变。方法健康雄性SD大鼠30只，质量220～250g，采用随机数字表法分为3组（n=10），假手术组（Sham组）、缺血再灌注组（IR组）和富氢水组（HS组）。Sham组仅暴露肝门，不夹闭动、静脉；IR组采用夹闭左、中叶肝肝动脉支60min（保留门静脉血流），然后恢复灌注，制备大鼠肝缺血再灌注模型；Hs组于手术前3天口服2％富氢水（40mL·kg^-1·d^-1），其余两组口服同等剂量生理盐水。各组于再灌注6h抽取下腔静脉血样并取肝组织，光镜下观察肝组织病理学变化。结果Sham组未见明显肝细胞坏死改变，肝小叶及肝细胞索结构完整，中央静脉、肝窦均正常。IR组肝脏再灌注后6h，肝细胞广泛变性，可见肝细胞灶性及片状坏死，肝中央静脉、肝窦间淤血，汇管区大量炎细胞聚集，部分肝小叶组织结构破坏。HS组与IR组相比，细胞坏死程度均减轻，肝细胞可见不同程度的水肿变性和空泡状变性，未见成片状细胞坏死，肝小叶结构完整，未见显著组织结构破坏，病理损害明显减轻。结论富氢水预处理可减轻大鼠肝脏的缺血再灌注损伤。Objective To investigate the pathology change of hydrogen-rich suline postcondi-tioning on hepatic ischemia-reperfu- sion injury in rats. Methods Thirty healthy male SD rats weighing 220-250g were randomly divided into 3 groups （n= 10 each） .. Shame operaton group（Sham group）, ischemia-reperfusion group （IR group） and hydrogemrich suline groups （ HS groups） In Sham group hepatic portal was only exposed but not iigated. IR group the left and middle lobes of hepatic pedicle,portal vein and hepatic artery were clamped for 60 min and then reperfused. HS groups 3 days before the surgery were received oral 2% rich hydrogen-rich water（40 mL·kg^-1·d^-1）. Blood samples were collected for measuring the activity of alanine transaminase（ALT）and aspartate transaminase（AST）in serm at 6h after reperfusion. Liver tissue samples were collected for pathologic examination. Results Sham group did not see obvious liver cell necrosis change,liver lobule and liver cells cable structure is complete, the central vein,hepatic sinus were normal. IR group liver 6 h after reperfusion,liver cell degeneration, hepatic cell patchy necrosis, central hepatic vein, hepatic sinus between blood, collect abbacy gathered a large number of inflammatory cells, part of the hepatic lobule structure damage. HS group compared with IR group, the extent of cell death are reducing, shows different degrees of edema, degeneration of liver cells and vacuolated degeneration,did not see the slice cell necrosis,hepatic lobule structure integrity,not seen significant organizational structure damage,significantly reduce the pathological damage. Conclusion Hydrogen-rich suline preconditioning can reduce hcpatic ischemia reperfusion injury in rats.

关 键 词：富氢水 预处理 肝脏再灌注损伤 病理改变 

分 类 号：R657.3[医药卫生—外科学]