L-精氨酸改性PVDF抗凝血膜的制备及血液相容性研究  

Preparation and blood compatibility of PVDF membrane modified with L-Arginine

作　　者：姜智旭 贾金兰[1] 石璐[1] 杨宁[1] 陈莉[1] 

机构地区：[1]天津工业大学材料科学与工程学院,天津300387

出　　处：《功能材料》2015年第23期23109-23114,共6页Journal of Functional Materials

基　　金：国家自然科学基金资助项目(21374078)

摘　　要：采用碱处理方法,以丙烯酸(AA)为中介,将L-精氨酸(ARG)共价接枝在聚偏氟乙烯(PVDF)膜表面,制备了PVDF-ARG膜。对改性前后的PVDF膜进行了X射线光电子能谱(XPS)、傅立叶变换红外光谱(FT-IR)表征,分析了改性前后膜表面的化学组成和结构变化,发现膜表面ARG的含量随ARG投料浓度的增加而增加。利用血小板粘附、蛋白吸附、复钙时间、溶血率等实验研究了改性膜的血液相容性。结果显示,经L-精氨酸改性的PVDF膜可有效地抑制血小板粘附、蛋白吸附,可降低溶血程度,延长复钙时间,并随着膜表面ARG含量的增加,改性膜的血液相容性更好。L-Arginine( ARG) was covalently immobilized onto the surface of poly( vinylid-ene fluoride)( PVDF) membrane that were prepared by the method of alkali treatment and using acrylic acid( AA) as the intermediary. The surface chemistry changes of modified films were characterized by X-ray photoelectron spectroscopy( XPS) and Fourier transform infrared( FT-IR) spectroscopy. It was found that the grafting degree of the ARG increases with the increase of ARG concentration in the reaction solution. The in vitro anticoagulant activities of the surface-modified PVDF membranes were evaluated by hemolytic test,and plasma recalcification time( PRT),protein adsorption,platelet adhesion. The results indicate that the PVDF membrane modified by ARG can suppress the protein adsorption,platelet adhesion,hemolysis rate and prolong the plasma recalcification time.

关 键 词：聚偏氟乙烯 L-精氨酸 丙烯酸 血液相容性 

分 类 号：TB34[一般工业技术—材料科学与工程]