基于上转换荧光共振能量转移检测  

Biosensor based on up-converting phosphors as energy donor for sensing biomolecules

作　　者：王玉洁[1] 罗灏[1] 张玉华[2] 何洁鑫 杨佐毅[1] 刘敬勇[1] 

机构地区：[1]广东工业大学环境科学与工程学院,广州510006 [2]香港科技大学土木与环境工程学系

出　　处：《功能材料》2015年第23期23115-23119,共5页Journal of Functional Materials

基　　金：人保部2012年度留学人员科技活动资助项目(12ZK0269);广州市环保局新技术新工艺开发资助项目(13ZK0424)

摘　　要：设计了一种基于分子信标的新型生物检测模型,以上转化发光纳米粒子(UCPs)作为FRET能量供体,利用分子信标茎环结构的优势,有效减小供受体之间距离,从而增加FRET能量转移效率,提高猝灭效率和生物检测灵敏度,并将该模型用于目标DNA和凝血酶检测,得到了令人满意的结果。此外,将这种灵敏且结构简单的模型用于复杂基质中凝血酶的检测,显示出UCPs优越的抗干扰性能。In this study,we design a new biosensor using up-converting phosphors( UCPs) as the energy donor for biomolecules sensing. The problem of the limited quenching efficiency was effectively resolved by constructing MB sensors in this work. Because of the stem-loop hairpin structure,it can reduce the donor-to-acceptor distance and hence significantly increased the energy transfer efficiency and improved the sensitivity. We apply this sensor in the detection of targetDNA and thrombin with satisfactory results obtained. What's more,the up-converting MB sensor in simple and flexible structure was used to monitor thrombin in complicated biological sample matrix,demonstrating the ability of anti-interference.

关 键 词：荧光共振能量转移 上转换荧光纳米材料 分子信标 生物 

分 类 号：O657.3[理学—分析化学]