有关双向电泳中植物蛋白制备的研究  

Study on Plant Protein Sample Preparing in Two-dimensional Electrophoresis

作　　者：程霞[1] 苏源[1] 窦玉敏[1] 刘开庆[1] 邓纲[2] 

机构地区：[1]昆明学院生命科学与技术系,云南昆明650214 [2]云南大学农学院,云南昆明650091

出　　处：《昆明学院学报》2015年第6期69-71,共3页Journal of Kunming University

基　　金：云南省教育厅资助项目(2014Y391);云南省高校特色生物资源开发与利用重点实验室开放基金和主任基金

摘　　要：通过蛋白组学技术研究生物体最终的执行功能是后基因组时代的重点之一.目前,双向电泳因成本低、分离效果好仍然是蛋白组学研究的一项经典技术.为了得到高质量的双向电泳图谱,首先需制备出蛋白含量高、杂质少、稳定性好的植物样品.因此,结合植物蛋白提取的原理和方法,对蛋白制备结果影响较大的细胞破碎、预分级、杂质去除、裂解液配置以及对蛋白的溶解等几方面提出了针对性的建议.It is one of the key focus in the post genome era that the final executive function of biology is studied through proteomics technolo-gy.At present,the two-dimensional electrophoresis is still a classic technology of proteomics research because of its low cost and good separa-tion effect.In order to get high quality map of two-dimensional electrophoresis,it is necessary to produce good plant samples with high protein content,less impurity and good stability.Combining the principle and method of plant protein extraction,we put forward the targeted suggestions that influence the result of the protein preparation,such as cell crushing,grading,impurity removal,lysis buffer content and so on.

关 键 词：双电电泳 蛋白质 电泳图谱 裂解液 

分 类 号：Q512[生物学—生物化学]