十溴联苯醚与邻苯二甲酸二(2-乙基)酯致雄性生殖损伤的研究  

The study of reproductive impairment of adolescent male ICR mice induced by BDE-209 and DEHP combined administration

作　　者：丁承辉[1] 翟金霞[1] 任征[1] 戴瑞雪[1] 林浩飞 李俊[1] 李玉荣[1] 

机构地区：[1]安徽医科大学公共卫生学院劳动卫生与环境卫生学系,安徽合肥230032

出　　处：《中华疾病控制杂志》2015年第10期1034-1037,共4页Chinese Journal of Disease Control & Prevention

基　　金：安徽省自然科学基金(1408085MH192);安徽医科大学博士基金(XJ201201)

摘　　要：目的探讨十溴联苯醚(decabromodiphenylether,BDE-209)与邻苯二甲酸二(2-乙基)酯(Bis(2-ethylhexyl)phthalate,DEHP)联合暴露致青春期雄性小鼠生殖损伤的表现。方法 4周龄青春期雄性ICR小鼠随机分为5组:A组为玉米油对照组;B组为100 mg/(kg·d)BDE-209+100 mg/(kg·d)DEHP组;C组为100 mg/(kg·d)BDE-209+300 mg/(kg·d)DEHP组;D组为300 mg/(kg·d)BDE-209+100 mg/(kg·d)DEHP组;E组为300mg/(kg·d)BDE-209+300 mg/(kg·d)DEHP组。小鼠经口染毒6周后处死,称取小鼠脏器重量并计算脏器系数,附睾尾部精子计数,采用放射免疫检测法检测小鼠血清睾酮浓度,采用免疫印迹实验检测小鼠睾丸睾酮合成酶的表达水平。结果与对照组相比,E组小鼠睾丸脏器系数降低(P<0.001),D、E组小鼠血清睾酮浓度显著降低(P=0.021,P=0.003),各联合暴露组小鼠附睾精子计数均降低(均有P<0.001),且小鼠睾丸类固醇激素合成急性调节蛋白St AR、胆固醇侧链裂解酶(P450scc)、17β-羟基类固醇脱氢酶(17β-HSD)合成表达量降低。结论 BDE-209和DEHP联合暴露,能够诱导青春期雄性ICR小鼠雄性生殖功能损伤。Objective To discuss the reproductive toxicity of BDE-209 and DEHP combined exposure to the adolescent male ICR mice. Methods The 4 weeks old adolescent male ICR mice were randomly divided into 5 groups： A： corn oil control group, B：100 mg/（kg · d） BDE-209 ＋ 100 mg/（kg·d） DEHP group; C：100 mg/（kg·d） BDE-209 ＋ 300 mg/（kg·d） DEHP group; D：300 mg/（kg·d） BDE-209 ＋100 mg/（kg·d） DEYIP group;and E：300 mg,/（kg·d） BDE-209 ＋300 mg,/（kg·d） DEHP group. The mice were executed 6 weeks after BDE-209 and DEHP combined exposure. The mice sperm＇ s number was recorded and the testosterone tested by radioimmunoassay. The levels of STAR, P450scc, and 1713-HSD expression in testicular were measured by Western blot. Results Compared with the control group, the E group mice testicular coefficient was significantly decreased （ P 〈 0. 001 ）. The D and E group mice Lg（ serum testosterone, 2. 14 ± 0. 84 ng/mL, 2. 06 ±0. 67 ng/mL respectively ） was decreased （P = 0. 021,P = 0. 003 ） and all the combined exposure groups mice sperm＇s number（ × 10^6/mL） was decreased （P 〈0. 001 ）. The STAR, P450scc and 17β- HSD expressions in testicular were down-regulated. Conclusions BDE-209 and DEHP combined exposure could induce reproductive impairment in the adolescent male ICR mice.

关 键 词：内分泌干扰物 生殖毒性 小鼠 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]