涤痰通瘀方干预急性脑出血大鼠肝组织IRAK-M表达变化的影响  

IRAK-M in liver during acute cerebral hemorrhage and effects of Ditantongyu measure

作　　者：王威[1] 许晓英[1] 马迪[1] 赵海滨[1] 唐杰[2] 张秀静[1] 王帅[1] 翟瑶瑶 张淑静[3] 

机构地区：[1]北京中医药大学第三附属医院,北京100029 [2]北京房山区中医医院,北京102400 [3]北京中医药大学,北京100029

出　　处：《吉林中医药》2015年第11期1155-1158,共4页Jilin Journal of Chinese Medicine

基　　金：国家自然科学基金(81373590)

摘　　要：目的研究急性脑出血大鼠肝组织中IRAK-M表达的变化,并探讨涤痰通瘀方剂对急性脑出血大鼠肝损害的干预作用。方法通过建立SD大鼠急性脑出血模型,并随机分为中药组(酒大黄、胆南星、石菖蒲、蒲黄、三七、水蛭)、脑出血组、假手术组、正常组,每组分为24、48、72 h 3个时间亚组,检测每组肝脏组织IRAKM蛋白表达水平及肝组织肿瘤坏死因子-α(TNF-α)水平,并观察肝组织HE染色结果。结果脑出血模型组在造模后各个时间点IRAK-M蛋白表达水平较假手术组及正常组均明显降低(P<0.05);中药治疗组的IRAKM蛋白表达水平较脑出血模型组明显升高(P<0.05),与假手术组、正常组相比略有降低;中药治疗组及脑出血模型组肝脏IRAK-M蛋白表达水平与组织TNF-α水平呈负相关。结论 IRAK-M蛋白表达水平升高可能是抑制脑出血后诱发的LPS胞内信号转导炎性级联反应的重要因素;涤痰通瘀方剂可加强IRAK-M激酶活性,从而抑制炎症因子的释放,减轻急性脑出血大鼠肝脏损伤的程度。Objective To investigate the change of expression of IRAK-M in liver during Acute Cerebral Hemorrhage, and discuss the protection mechanism of Ditantongyu Measure. Methods The model of acute cerebral hemorrhage was established. The animals were randomly divided into three groups ： TCM treatment group （ the rhubarb, bravery south star, stone calamus, cattail pollen, notoginseng, leech ） , cerebral hemorrhage group, sham surgery group, blank control group. And each group was further divided into 3 time-point sub-groups of 24,48 and 72 h. The expression level of hepatic IRAK-M protein was measured, the hepatic content of tumor necrosis factor-alpha （TNF-α） were detected, and HE staining results of liver tissue are observed. Results Compared with Group S and Group B, the expres- sion levels of IRAK-M protein at each time-point post-modeling in both Groups T had markedly reduced （ P 〈 0. 05 ）. Groups T elevated markedly compared with Group C （P 〈 0.05 ）, but it decreased in Group S and Group B. The expression level of hepatic IRAK-M protein in Group T and Group C was negatively correlated with the changes of hepat- ic content of TNF-α. Conclusion Increase of IRAK-M protein expression may be an important factor in the inhibition of after cerebral hemorrhage induced intracellular signal transduction of the inflammatory cascade reaction ; Ditantongyu Measure can enhance the activity of IRAK-M, and it can restrain release of inflammatory factor, thus alleviating liver injury of rats with acute cerebral hemorrhage.

关 键 词：白细胞介素-1受体相关激酶-M 急性脑出血 肝损害 涤痰通瘀法 肿瘤坏死因子-α 

分 类 号：R285.5[医药卫生—中药学]